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PXD050495

PXD050495 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics and metabolic burden analysis to understand the impact of recombinant protein production on host cells
DescriptionRecombinant protein production (RPP) using Escherichia coli as an expression host is highly efficient, however, it also in¬ leads to strong host cell metabolic burden. Energy and biomass precursors are withdrawn from the host’s metabolism as they are required for plasmid replication, heterologous gene expression and protein production. In this study, we aim to investigate the effect of early and growth phase induction point on RPP. We have analysed and compared the proteomics data for growth-and protein production-phase to understand the cellular dynamics and recombinant protein-dependent product formation in process with respect to the two different hosts (M15 and DH5⍺) and medium (minimal and complex) cultures. Differential gene analysis shows that significant changes in carbohydrate metabolism, transport metabolism, nucleotide metabolism, amino acid metabolism and energy metabolism. Comprehension of the obtain results suggest that growth inhibition does not showing effect on recombinant protein and product yield but there is an changes in intracellular nucleobases, translation, transcription and cellular metabolism which leads to the degradation and instability during product formation. Overall, deeper knowledge of the cellular behaviour during recombinant protein production can be used to better exploit omics data with the goal of rational strain development.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:34:21.598.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD050495
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGirish Halemirle Rajacharya
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-09 08:54:18ID requested
12024-03-23 06:21:16announced
22024-10-22 06:34:22announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD050495;
Keyword List
submitter keyword: recombinant protein production, 39proteomics, metabolic burden, gene expression, acyl-(acyl carrier protein) reductase (AAR), post-induction growth phases
Contact List
Syed Shams Yazdani
contact affiliationMicrobial Engineering Group, ICGEB, India
contact emailshams@icgeb.res.in
lab head
Girish Halemirle Rajacharya
contact affiliationIndian Institute of Technology, Delhi
contact emailhr.girish014@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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