PXD050490

PXD050490 is an original dataset announced via ProteomeXchange.

Title	Functional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (subcellular localization and relocalization analysis)
Description	The subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylation for cellular functions, farnesyltransferase inhibitors (FTIs) including tipifarnib were developed and are being tested in oncology clinical trials. The current lack of knowledge on the functional impact of FTIs on hundreds of farnesylation substrates limits response prediction and their use in cancer precision medicine. Here, we report a global functional proteomics investigation of farnesylation and the impact of tipifarnib in multiple lung cancer cell lines. This dataset includes global mapping of protein localization using our SubCellBarCode method, and analyses of tipifarnib-dependent protein relocalization.
HostingRepository	PRIDE
AnnounceDate	2025-05-19
AnnouncementXML	Submission_2025-05-19_05:33:41.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgios Mermelekas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-08 12:55:27	ID requested
⏵ 1	2025-05-19 05:33:42	announced

10.1016/j.isci.2025.111864;

Pan Y, Berkovska O, Marathe S, Mermelekas G, Gudoityte G, Wolide AD, Arslan T, Seashore-Ludlow B, Lehti, ö J, Orre LM, Functional-proteomics-based investigation of the cellular response to farnesyltransferase inhibition in lung cancer. iScience, 28(2):111864(2025) [pubmed]

submitter keyword: proteomics, tipifarnib,Lung cancer, human, farnesylation, subcellular, HiRIEF, cell line, LC-MS/MS

Lukas Orre
contact affiliation	Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden
contact email	Lukas.orre@ki.se
lab head
Georgios Mermelekas
contact affiliation	Karolinska Institutet
contact email	georgios.mermelekas@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050490

PRIDE project URI

• PRIDE
  1. PXD050490
     1. Label: PRIDE project
     2. Name: Functional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (subcellular localization and relocalization analysis)