PXD050461

PXD050461 is an original dataset announced via ProteomeXchange.

Title	Blood Proteome profiling for protein biomarker discovery in Broilers
Description	Analysis of the blood proteome allows identification of proteins related to changes upon certain physiological conditions. The pathophysiology of necrotic enteritis (NE) has been extensively studied. While intestinal changes have been very well documented, data addressing NE-induced alterations in the blood proteome are scant, although these might have merit in diagnostics. Considering recent technological advancements in proteomics and pressing need for tools to access gut health, the current study employs mass-spectrometry (MS) proteomics to identify biomarkers for gastrointestinal health of broilers chickens. Untargeted proteomics investigation was conducted on chicken blood plasma in animals under NE challenge. Two MS-strategies were used for analysis: DDA (Data Dependent Acquisition) and DIA (Data Independent Acquisition). DIA showed superior completeness and quantification of the acquired data, despite high degree of agreement in identification and quantification between both approaches. Identified differentially expressed proteins shared by DDA and DIA represent responses of animals to infection and may serve as potential biomarkers. Experimental validation through ELISA immunoassays for selected regulated proteins confirmed medium-to-high levels of inter-protein correlation, along with positive correlation between the methods. Functional analysis showed enhanced defense, immune, and acute phase responses, alongside reduced signaling, regulatory, and cell adhesion activities in infected animals.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_06:16:07.545.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050461
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Teresa Mendes Maia
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-07 15:57:20	ID requested
⏵ 1	2025-05-13 06:16:08	announced

10.1038/s41598-025-97783-w;

Tretiak S, Maia TM, Van Haver D, Staes A, Devos S, Rijsselaere T, Goossens E, Van Immerseel F, Impens F, Antonissen G, Blood proteome profiling for biomarker discovery in broilers with necrotic enteritis. Sci Rep, 15(1):12895(2025) [pubmed]

10.6019/PXD050461;

submitter keyword: proteomics, DIA, gastrointestinal health, necrotic enteritis,broiler chicken, DDA

Francis Impens
contact affiliation	VIB-UGent Center for Medical Biotechnology UGent Department of Biomolecular Medicine Technologiepark-Zwijnaarde 75 B9052 Gent Belgium
contact email	Francis.Impens@vib-ugent.be
lab head
Teresa Mendes Maia
contact affiliation	VIB Proteomics Core
contact email	teresa.maia@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050461
     1. Label: PRIDE project
     2. Name: Blood Proteome profiling for protein biomarker discovery in Broilers