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PXD050458

PXD050458 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnveiling the Proteome of the Fasting Heart: Insights into HIF-1 Pathway Regulation.
DescriptionFasting is a common dietary intervention known for its protective effects against metabolic and cardiovascular diseases. While its effects are mostly systemic, understanding tissue-specific changes in the heart is crucial for the identification of the mechanisms underlying fasting-induced cardioprotection. In this study, we performed a proteomic analysis to elucidate the proteome of the fasting heart. Our investigation identified a total of 4,652 proteins, with 127 exhibiting down-regulation and 118 showing up-regulation after fasting. Annotation analysis highlighted significant changes in processes such as lipid metabolism, the peroxisome pathway, and reactive oxygen species metabolism. Notably, the HIF-1 signaling pathway emerged as one of the focal points, with five affected proteins identified. Further experiments demonstrated down-regulation of HIF-1α at both transcript and protein levels. Intriguingly, while gene expression of Egln3 decreased, its protein product PHD3 remained unaffected by fasting. These findings underscore the regulation of the well-established cardioprotective HIF-1 signaling within the heart during 3-day fasting.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_07:00:55.866.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarek Vrbacky
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmethylthiolated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-07 13:39:10ID requested
12024-10-08 14:37:18announced
22024-10-22 07:00:56announced2024-10-22: Updated project metadata.
Publication List
10.3389/FPHYS.2024.1462014;
Keyword List
submitter keyword: fasting, HIF-1, proteome,heart, PHD3
Contact List
Marketa Hlavackova
contact affiliationInstitute of Physiology of the Czech Academy of Sciences Laboratory of Developmental Cardiology
contact emailMarketa.Hlavackova@fgu.cas.cz
lab head
Marek Vrbacky
contact affiliationCzech Academy of Sciences
contact emailproteom.krc@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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