PXD050438

PXD050438 is an original dataset announced via ProteomeXchange.

Title	Global quantitative MS proteome analysis of in vitro activated murine primary B cells of Dis3G766R/+ and Dis3+/+ genotypes
Description	DIS3 is a ribonucleolytic subunit of the exosome complex responsible for RNA degradation in the nucleus, predominantly affecting steady state levels of non-coding transcripts. The effect of the mild mutant Dis3 G766R allele, clinically related t multiple myeloma, in a heterozygous state on protein-coding transcripts is negligible. In this experiment, we assayed the proteome of in vitro activated primary B cells 72 hours after their activation with LPS (20 µg/ml) and IL-4 (20 ng/ml). TMT-based global quantification of protein levels was performed in Dis3G766R/+ and Dis3+/+ samples in triplicates per condition. This dataset is directly related to a transcriptomic analysis available under a GEO accession number GSE155631 containing RNA-seq and DRIP-seq data of corresponding samples
HostingRepository	PRIDE
AnnounceDate	2026-02-13
AnnouncementXML	Submission_2026-02-13_04:53:45.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrzej Dziembowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-07 04:52:57	ID requested
⏵ 1	2026-02-13 04:53:45	announced

Dataset with its publication pending

submitter keyword: mouse, Dis3G766R/+ and Dis3+/+ genotypes, LPS and IL-4 activation, B cells

Andrzej Dziembowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	adziembowski@iimcb.gov.pl
lab head
Andrzej Dziembowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	adziembowski@iimcb.gov.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD050438

PRIDE project URI

• PRIDE
  1. PXD050438
     1. Label: PRIDE project
     2. Name: Global quantitative MS proteome analysis of in vitro activated murine primary B cells of Dis3G766R/+ and Dis3+/+ genotypes