PXD050436

PXD050436 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of Proteomics and lipidomics profiles reveal the fat deposition and meat quality in Duroc×Guangdong small spotted pig
Description	This study aims to explore the important factors affecting the characteristics of different parts of pork. Lipidomics and proteomics methods were used to analyze DAL (differential lipids) and DAPs (differential proteins) in five different parts(longissimus dorsi, belly meat, loin, forelegs and buttocks))of Duhua pig(Duroc×Guangdong small spotted pig)，to identify potential pathways affecting meat quality, investigating fat deposition in pork and its lipid-protein interactions. The results show that TG (triglyceride) is the lipid subclass with the highest proportion in muscle, and the pathway with the most significantly enriched lipids is GP. DAP clustered on several GO terms closely related to lipid metabolism and lipogenesis (lipid binding, lipid metabolism, lipid transport, and lipid regulation). In KEGG analysis, there are two main DAP aggregation pathways related to lipid metabolism, namely Fatty acid degradation and oxidative phosphorylation. In PPI analysis, we screened out 31 core proteins, among which NDUFA6, NDUFA9 and ACO2 are the most critical. PC (phosphatidylcholine) is regulated by SNX5, THBS1, ANXA7, TPP1, CAVIN2, and VDAC2 in the phospholipid binding pathway. TG is regulated by AUH/HADH/ACADM/ACADL/HADHA in the lipid oxidation and lipid modification pathways. Potential biomarkers are rich in SFA, MUFA and PUFA respectively, the amounts of SFA, MUFA and PUFA in the lipid measurement results are consistent with the up- and down-regulation of potential biomarker lipids. This study clarified the differences in protein and lipid compositions in different parts of Duhua pigs and provided data support for revealing the interactions between pork lipids and proteins. These findings provide contributions to the study of intramuscular fat deposition in pork from a genetic and nutritional perspective.
HostingRepository	PRIDE
AnnounceDate	2024-04-07
AnnouncementXML	Submission_2024-04-07_00:28:04.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuosui Wu
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 23:42:03	ID requested
⏵ 1	2024-04-07 00:28:05	announced

10.3389/FVETS.2024.1361441;

submitter keyword: fat deposition

lipidomics

proteomics

metabolic pathways

Duroc×Guangdong small spotted pig

Zhuosui Wu
contact affiliation	Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, 510640, China
contact email	wzs981207@gmail.com
lab head
Zhuosui Wu
contact affiliation	Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, 510640, China
contact email	wzs981207@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050436

PRIDE project URI

• PRIDE
  1. PXD050436
     1. Label: PRIDE project
     2. Name: Integrative analysis of Proteomics and lipidomics profiles reveal the fat deposition and meat quality in Duroc×Guangdong small spotted pig