PXD050432

PXD050432 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mechanism of isorhynchophylline in lipopolysaccharide-induced acute lung injury based on proteomic technology
Description	Abstract Isorhynchophylline, a tetracyclic indole alkaloid, has anti-inflammatory and antioxidant activities against cardiovascular diseases and central nervous system disorders. Acute lung injury (ALI) is a manifestation of inflammation concentrated in the lungs and has a high incidence rate and mortality. Here, we established a mouse model of ALI and observed the effects of isorhynchophylline. Proteomic results showed that 5727 proteins were detected in mouse lung tissues, and 16 proteins were screened out. Isorhynchophylline could reverse the trend of these differential proteins. In addition, isorhynchophylline can act on integrin alpha M to reduce neutrophil recruitment and thereby produce anti-inflammatory effects and may suppress neutrophil migration through the leukocyte transendothelial migration pathway. TUNEL and RT-PCR experiments revealed that LPS-induced ALI in mice increases the apoptosis of lung tissues, damage to alveolar epithelial cells and levels of inflammatory factors. Treatment with isorhynchophylline can repair tissues, improve lung tissue pathology and reduce lung inflammation.
HostingRepository	PRIDE
AnnounceDate	2024-06-23
AnnouncementXML	Submission_2024-06-23_01:20:28.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaru Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 19:36:25	ID requested
⏵ 1	2024-06-23 01:20:28	announced

Publication List

10.3389/fphar.2024.1397498;
Li Y, Xing J, Qin L, Zhang C, Yang Z, Qiu M, Mechanism of isorhynchophylline in lipopolysaccharide-induced acute lung injury based on proteomic technology. Front Pharmacol, 15():1397498(2024) [pubmed]

10.3389/fphar.2024.1397498;

Li Y, Xing J, Qin L, Zhang C, Yang Z, Qiu M, Mechanism of isorhynchophylline in lipopolysaccharide-induced acute lung injury based on proteomic technology. Front Pharmacol, 15():1397498(2024) [pubmed]

Keyword List

submitter keyword: Isorhynchophylline；Acute lung injury；Proteomic technology
TUNEL
RT-PCR
Nano-LC-MS/MS

submitter keyword: Isorhynchophylline；Acute lung injury；Proteomic technology

TUNEL

RT-PCR

Nano-LC-MS/MS

Contact List

Qiu Min
contact affiliation	Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China
contact email	102006095@btmc.edu.cn
lab head
Yaru Li
contact affiliation	Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China
contact email	liyaru2020@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD050432
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD050432

PRIDE project URI

Repository Record List

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