PXD050424

PXD050424 is an original dataset announced via ProteomeXchange.

Title	Understanding with environmental proteomics the underlying processes triggered by ornamental lighting on phototrophic biofilms growing on stone-built heritage.
Description	Recent and ongoing studies evidence that certain LED lights, suitable for illuminating the urban fabric, avoiding primary colours such as blue, green, and red, may halt the biological colonization on stone monuments, mainly caused by algae and cyanobacteria forming subaerial biofilms (SABs). However, the light-triggered mechanisms that cause changes in the growth of the phototrophic species is so far unknown. An environmental proteomic approach could shed light on this aspect, providing information on the changes on SAB’s metabolism under the stress inflicted by different ornamental lighting. Here, laboratory-made SABs mainly composed of Chlorophyta, and at lesser extent Streptophyta and Cyanophyta, were subjected to three lighting conditions for monuments: cool white, warm white and, amber+green (with biostatic effect and under trial) A control without light (i.e., darkness) was also included for comparison. Cool white and warm white illumination severely affected SAB’s proteome reducing their total peptide identifications being the extent of this reduction dependent on the organisms’ genera. The photo-damaging effect of amber+green on the biofilm metabolism was clarified, revealing its negative impact on the photosystems I and II and antenna pigments production, and its triggering effect on the protein metabolism (synthesis, folding, and degradation). This research provides for the first-time a thorough description of the proteomic changes induced by lighting on SABs colonising illuminated monuments in urban areas. The insights gained will help to better design the lighting of monuments, with the inclusion of ornamental lighting as one of the elements to be taken into account in the preventive conservation of the built heritage.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_06:03:49.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alba Trueba-Santiso
SpeciesList	scientific name: Cyanobacteria; NCBI TaxID: 1117; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 15:54:33	ID requested
⏵ 1	2025-05-13 06:03:50	announced

M, é, ndez A, Sanmart, í, n P, Balboa S, Trueba-Santiso A, Environmental Proteomics Elucidates Phototrophic Biofilm Responses to Ornamental Lighting on Stone-built Heritage. Microb Ecol, 87(1):147(2024) [pubmed]

10.1007/s00248-024-02465-1;

submitter keyword: environmental proteomics

lighting treatment

monumental illumination

Scenedesmus, Tetradesmus and Chlorella

stone heritage

subaerial biofilm (SAB).

Prof. Patricia Sanmartin Sánchez
contact affiliation	GEMAP (GI-1243), Departamento de Edafoloxía e Química Agrícola, Facultade de Farmacia, Universidade de Santiago de Compostela, Santiago de Compostela, 15782, Spain. CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
contact email	patricia.sanmartin@usc.es
lab head
Alba Trueba-Santiso
contact affiliation	University of Santiago de Compostela
contact email	albamaria.trueba@usc.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050424
     1. Label: PRIDE project
     2. Name: Understanding with environmental proteomics the underlying processes triggered by ornamental lighting on phototrophic biofilms growing on stone-built heritage.