PXD050411 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | proteomic and metabolomic profiling of methicillin resistant and methicillin sensitive Staphylococcus aureus using a simultaneous extraction protocol |
| Description | understanding the biology of methicillin resistant staphylococcus aureus (MRSA) is crucialto unlocking insights for new targets in the fight against this pathogen, there is howeverlimited reports of methadological approaches for carrying proteomic and metabolomic profiling in S.aureus. Therefore, we describe the use of a dual-functionality methanol extraction method for the concurrent extraction of protein and metabolites from S.aureus and reporton the comparative analysis of the proteomic and metabolomic profiles of MRSA versus methicillin sensitive S. aureus (MSSA). Using a reference strain from MRSA and MSSA, we first compared the MRSA proteome extracted using the methanol method to the one from the traditionally used urea method. Then using the methanol extraction method, we compared the proteome and metabolome of MRSA versus MSSA. Through this study, we demonstrated the effectivnessof the methanol-based-dual-extraction method, providing simultaneous insights into the proteomic and metabolomic landscapes of S.aureus strains, demonstrating the utility of proteomic and metabolomic profiling for elucidating the biological basis of antimicrobial resistance |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_06:54:14.905.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Core Facility |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-03-06 10:54:50 | ID requested | |
| 1 | 2024-08-10 12:18:13 | announced | |
| ⏵ 2 | 2024-10-22 06:54:18 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.3389/fmicb.2024.1402796; |
| Boucherabine S, Giddey A, Nassar R, Al-Hroub HM, Mohamed L, Harb M, Soares NC, Senok A, using a simultaneous extraction protocol. Front Microbiol, 15():1402796(2024) [pubmed] |
Keyword List
| submitter keyword: MRSA, Dual-functionality,Staphylococcus aures, metabolomics. proteomics |
Contact List
| Abiola Senok |
|---|
| contact affiliation | College of Medicine, Mohammed Bin Rashid University of Medicine and Health Science |
| contact email | Abiola.Senok@mbru.ac.ae |
| lab head | |
| Core Facility |
|---|
| contact affiliation | Sharjah Institute for Medical Research, University of Sharjah |
| contact email | tims-tof@sharjah.ac.ae |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050411
- Label: PRIDE project
- Name: proteomic and metabolomic profiling of methicillin resistant and methicillin sensitive Staphylococcus aureus using a simultaneous extraction protocol
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