PXD050392

PXD050392 is an original dataset announced via ProteomeXchange.

Title	Glycosylation analysis of SARS-CoV-2 spike protein
Description	The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has globally impacted on public health. Since the glycosylation of viral envelope glycoproteins is deeply associated with their immunogenicity, intensive studies on the glycans of the glycoprotein of SARS-CoV-2, the spike (S) protein, have been conducted. Here, we conducted intensive glycoproteomic analyses of the SARS-CoV-2 S protein of ancestral and gamma variant strains using a combinatorial approach with two different technologies: mass spectrometry (MS) and lectin microarray. Our unique MS1-based glycoproteomic technique, Glyco-RIDGE, in addition to MS2-based Byonic search, identified 1,448 (ancestral strain) and 1,785 (gamma variant strain) site-specific glycan compositions, respectively. An asparagine at amino acid position 20 (N20) was mainly glycosylated within two successive potential glycosylation sites, N17 and N20, of the gamma variant S protein; however, we newly found low-frequency glycosylation at N17. Our novel approaches, glycostem mapping and glycoleaf scoring, also illustrated the moderately branched/extended, highly fucosylated, and less sialylated nature of the glycoform of S proteins. The subsequent lectin microarray analysis emphasized intensive end-capping of glycans by Lewis fucoses, which complemented the glycoproteomic features. These results illustrate high-resolution glycoproteomic features of the SARS-CoV-2 S protein, contributing to vaccine design and the understanding of viral protein synthesis.
HostingRepository	jPOST
AnnounceDate	2025-03-06
AnnouncementXML	Submission_2025-03-10_02:08:56.155.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroaki Sakaue
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	S-carboxamidomethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln); L-methionine sulfoxide; Ammonia-loss; unknown modification
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 01:34:51	ID requested
⏵ 1	2025-03-10 02:08:56	announced

Dataset with its publication pending

submitter keyword: SARS-CoV-2, COVID-19, spike protein, glycosylation, glycoproteomics

Takahiro Hiono
lab head
Hiroaki Sakaue
contact affiliation	National Institute of Advanced Industrial Science and Technology (AIST)
dataset submitter

jPOST dataset URI

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