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PXD050376

PXD050376 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRab30 regulates lipid homeostasis in the hepatocyte during fasting
DescriptionIn order to facilitate inter-tissue communication and exchange of proteins, lipoproteins, and metabolites with the circulation, hepatocytes have an intricate and efficient intracellular trafficking system regulated by small Rab GTPases. Rab30, a putative Golgi-localized Rab GTPase, is induced in the mouse liver by fasting and its expression is further amplified in liver-specific carnitine palmitoyltransferase 2 knockout mice (Cpt2L-/-) that lack the ability to oxidize fatty acids in a Pparα-dependent manner. Live-cell super-resolution imaging and biochemical in vivo proximity labeling demonstrated that Rab30-marked vesicles are highly dynamic and interact with proteins throughout the secretory pathway. Rab30 whole-body, liver-specific, and Rab30;Cpt2 liver-specific double knockout (DKO) mice are viable and display intact Golgi ultrastructure. However, the loss of Rab30 in Rab30;Cpt2 DKO mice suppresses serum dyslipidemia observed in Cpt2L-/- single knockout mice. Corresponding with decreased serum triglyceride and cholesterol levels, Rab30;Cpt2 DKO mice exhibit decreased circulating but not hepatic ApoA4 protein, indicative of a trafficking defect. Together, these data suggest a role for Rab30 in the selective sorting of lipoproteins to influence hepatocyte and circulating triglyceride levels particularly during times of excessive lipid burden.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:38:07.748.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD050376
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichael Wolfgang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListbiotinylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-05 12:53:54ID requested
12024-05-04 10:48:04announced
22024-10-22 06:38:08announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD050376;
Keyword List
submitter keyword: Rab GTPases
liver
Ppara
lipid metabolism
fasting
Contact List
Michael J. Wolfgang
contact affiliationDepartment of Physiology, The Johns Hopkins University School of Medicine
contact emailmwolfga1@jhmi.edu
lab head
Michael Wolfgang
contact affiliationJohns Hopkins University School of Medicine
contact emailmwolfga1@jhmi.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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