PXD050376

PXD050376 is an original dataset announced via ProteomeXchange.

Title	Rab30 regulates lipid homeostasis in the hepatocyte during fasting
Description	In order to facilitate inter-tissue communication and exchange of proteins, lipoproteins, and metabolites with the circulation, hepatocytes have an intricate and efficient intracellular trafficking system regulated by small Rab GTPases. Rab30, a putative Golgi-localized Rab GTPase, is induced in the mouse liver by fasting and its expression is further amplified in liver-specific carnitine palmitoyltransferase 2 knockout mice (Cpt2L-/-) that lack the ability to oxidize fatty acids in a Pparα-dependent manner. Live-cell super-resolution imaging and biochemical in vivo proximity labeling demonstrated that Rab30-marked vesicles are highly dynamic and interact with proteins throughout the secretory pathway. Rab30 whole-body, liver-specific, and Rab30;Cpt2 liver-specific double knockout (DKO) mice are viable and display intact Golgi ultrastructure. However, the loss of Rab30 in Rab30;Cpt2 DKO mice suppresses serum dyslipidemia observed in Cpt2L-/- single knockout mice. Corresponding with decreased serum triglyceride and cholesterol levels, Rab30;Cpt2 DKO mice exhibit decreased circulating but not hepatic ApoA4 protein, indicative of a trafficking defect. Together, these data suggest a role for Rab30 in the selective sorting of lipoproteins to influence hepatocyte and circulating triglyceride levels particularly during times of excessive lipid burden.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:07.748.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050376
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Wolfgang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-05 12:53:54	ID requested
1	2024-05-04 10:48:04	announced
⏵ 2	2024-10-22 06:38:08	announced	2024-10-22: Updated project metadata.

10.6019/PXD050376;

submitter keyword: Rab GTPases

liver

Ppara

lipid metabolism

fasting

Michael J. Wolfgang
contact affiliation	Department of Physiology, The Johns Hopkins University School of Medicine
contact email	mwolfga1@jhmi.edu
lab head
Michael Wolfgang
contact affiliation	Johns Hopkins University School of Medicine
contact email	mwolfga1@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050376
     1. Label: PRIDE project
     2. Name: Rab30 regulates lipid homeostasis in the hepatocyte during fasting