PXD050374

PXD050374 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles of Janthinobacterium lividum as violacein carriers in melanoma cell treatment.
Description	Violacein, a bisindole derivative produced, among others, by bacteria of the genus Janthinobacterium and Chromobacterium, is a compound with proven antimicrobial, anticancer and immunomodulatory properties. The anti-cancer activity of violacein has been tested on many cancer cells, including lung cancer cells, colorectal adenoma cells, acute myeloid leukaemia cells and melanoma cells. Interestingly, in a mouse model, a large difference in the response of cancerous and non-cancerous cells to violacein was observed, further highlighting the therapeutic potential of this compound. In addition, violacein has been observed to reduce the invasive potential of melanoma cells and has a much more potent anticancer effect than temozolomide, a drug used to treat melanoma. Violacein can be isolated from the bacterial cell pellet using solvents, and this metabolite is also secreted into the medium in a form enclosed in extracellular vesicles (EVs), in which the normally insoluble violacein remains suspended in water. The project focuses on the development of an efficient method of violacein application based on the use of EVs. Violacein-containing EVs were isolated from the culture of Janthinobacterium sp. and characterised by their appearance, size and protein composition. The activity of isolated EVs containing violacein was compared with the activity of violacein traditionally isolated from cells using solvents against four skin cell lines (cancerous and non-cancerous)
HostingRepository	PRIDE
AnnounceDate	2024-12-10
AnnouncementXML	Submission_2024-12-10_03:22:02.852.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050374
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ewa Sitkiewicz
SpeciesList	scientific name: Janthinobacterium lividum; NCBI TaxID: 29581;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-05 09:59:26	ID requested
⏵ 1	2024-12-10 03:22:03	announced

10.6019/PXD050374;

10.1007/S00253-024-13358-1;

submitter keyword: violacein, Janthinobacterium sp., extracellular vesicles, melanoma, EVs, drug carriers

Małgorzata Milner-Krawczyk
contact affiliation	Chair of Drug and Cosmetics Biotechnology, Warsaw University of Technology, Warsaw, Poland
contact email	malgorzata.krawczyk@pw.edu.pl
lab head
Ewa Sitkiewicz
contact affiliation	Mass Spectrometry Laboratory Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	ewa@ibb.waw.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050374
     1. Label: PRIDE project
     2. Name: Extracellular vesicles of Janthinobacterium lividum as violacein carriers in melanoma cell treatment.