PXD050370

PXD050370 is an original dataset announced via ProteomeXchange.

Title	Decontamination enhances palaeoproteomic analysis of a Pleistocene hominin tooth from Khudji, Tajikistan
Description	Contamination is a potential issue in palaeoproteomic studies of human evolution, as it may mask low-abundance endogenous proteins and introduce modern variation into the reconstructed ancient proteome. This contamination may stem from many sources, such as the burial environment, storage facilities, and subsequent handling. Here, we apply a bleach decontamination protocol to a Pleistocene hominin tooth from the archaeological site of Khudji, Tajikistan, which was found to be heavily contaminated with skin-derived proteins during initial analysis. Bleach treatment removed nearly all contaminating proteins from the dentine proteome, without damaging endogenous proteins. Finally, phylogenetic analysis of the reconstructed proteome showed that the tooth likely stems from a Neanderthal.
HostingRepository	PRIDE
AnnounceDate	2025-04-24
AnnouncementXML	Submission_2025-04-24_00:26:28.710.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050370
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zandra Fagernäs
SpeciesList	scientific name: Homo; NCBI TaxID: 9605;
ModificationList	hydroxylated proline; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-05 07:40:08	ID requested
⏵ 1	2025-04-24 00:26:29	announced

10.1016/J.JAS.2025.106228;

10.6019/PXD050370;

submitter keyword: Decontamination, Middle Palaeolithic,Palaeoproteomics, Archaeology, Neanderthal

Frido Welker
contact affiliation	Globe Institute, University of Copenhagen, Denmark
contact email	frido.welker@sund.ku.dk
lab head
Zandra Fagernäs
contact affiliation	Globe Institute, University of Copenhagen
contact email	zandra.fagernas@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD050370

PRIDE project URI

• PRIDE
  1. PXD050370
     1. Label: PRIDE project
     2. Name: Decontamination enhances palaeoproteomic analysis of a Pleistocene hominin tooth from Khudji, Tajikistan