PXD050367 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Complete list of canonical post-transcriptional modifications in Bacillus subtilis ribosome. |
Description | Using bottom-up oligonucleotide LC-MS/MS in combination with stable isotope labeling, chemical derivatization, and bioinformatics variable modification search, a total of 25 modifications were mapped to the sequences of 16S and 23S RNA in B. subtilis. 10 modification sites, previously identified by others using alternative methods were independently confirmed using targeted MS/MS data analysis. Modification types, namely base/ribose methylation, pseudouridines and dihydrouridine, and modification positions are fully consistent with prior experimental evidence and agree well with modifications that were annotated in E. coli and other bacteria species. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:20:30.816.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anna Popova |
SpeciesList | scientific name: Bacillus subtilis PY79; NCBI TaxID: 1415167; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
ModificationList | methylated residue; dimethylated residue |
Instrument | impact II; 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-05 07:19:51 | ID requested | |
⏵ 1 | 2024-10-08 11:20:31 | announced | |
Publication List
Keyword List
submitter keyword: pseudouridine, base and ribose methylations, RNA modifications,ribosomal RNA |
Contact List
James Williamson |
contact affiliation | Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA |
contact email | jrwill@scripps.edu |
lab head | |
Anna Popova |
contact affiliation | Scripps Research |
contact email | popova@scripps.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050367
- Label: PRIDE project
- Name: Complete list of canonical post-transcriptional modifications in Bacillus subtilis ribosome.