PXD050341

PXD050341 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of Protein Cysteine Acylation Using a Modified Suspension Trap (Acyl-Trap)
Description	Mouse brain and MLE-12 lysates were alkylated with N-ethylmaleimide, precipiated with methanol and trapped on a pyridyl disulfide quartx (PDQ) suspension trap. Protein were washed and incubated with -/+hydroxylamine and trypsin, and unbound peptides were labeled with TMTPro reagents on C18 StageTips. Bound peptides were labeled with TMTPro reagents on PDQ traps, eluted with DTT and alkylated with iodoacetamide followed by StageTip cleanup. Peptides were analyzed by nanoLC-MS/MS using a 90 min gradient in duplicate. MS/MS used a SPS-MS3 (or FAIMS-SPS-MS3) method as indicated in metadata. Raw files were converted to .mzml and analyzed using FragPipe.
HostingRepository	MassIVE
AnnounceDate	2024-03-19
AnnouncementXML	Submission_2024-03-19_12:18:57.914.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	TMTpro; Carbamidomethyl; N-ethylmaleimide derivatized cysteine; Acetyl; N-myristoylglycine
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-04 17:53:12	ID requested
⏵ 1	2024-03-19 12:18:58	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: palmitoylation, S-acylation

submitter keyword: palmitoylation, S-acylation

Contact List

Michael T. Forrester
contact affiliation	Duke University
contact email	michael.forrester@duke.edu
lab head
Matthew W. Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094234/