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PXD050334

PXD050334 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHelicobacter pylori gamma-glutamyltransferase relates to proteomic adaptations important for colonization
DescriptionHelicobacter pylori γ-glutamyltransferase (gGT) is a conserved virulence factor promoting bacterial colonization and immune tolerance. Although some studies addressed potential mechanisms explaining its function, the supportive role of gGT for in-vivo colonization remains unclear. In addition, it is unknown how differences in gGT expression observed for different strains may lead to compensatory mechanisms to ensure infection and persistence. Hence, it is of high importance to understand more profoundly the in-vivo function of this enzyme. In this study, we assessed acid survival under physiologic conditions mimicking the human gastric fluid and elevated the pH in the murine stomach prior to H. pylori infection to link gGT-mediated acid resistance to colonization. Furthermore, by comparing protein expression levels between wildtype and gGT-deficient H. pylori isolates before and after infecting mice, we systematically investigated proteomic adaptations of gGT-deficient bacteria during infection. Our data indicate that gGT plays a crucial role in sustaining urease activity in acidic environments, thereby supporting bacterial survival and successful colonization. The absence of gGT triggers the expression of proteins involved in nitrogen and iron metabolism, indicating a potential functional role of gGT in these metabolic pathways. Moreover, we observed an impaired growth of gGT-deficient strains in iron-restricted medium. In addition, the absence of gGT during infection leads to a significant overexpression of H. pylori adhesins and flagellar proteins, resulting in increased motility and adhesion capacity. In summary, we identified gGT-dependent mechanisms conferring a growth advantage to the bacterium in the gastric environment, which renders gGT a valuable target for the development of new treatments against H. pylori infection.
HostingRepositoryPRIDE
AnnounceDate2025-04-08
AnnouncementXMLSubmission_2025-04-08_05:05:23.631.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNina Bach
SpeciesList scientific name: Helicobacter pylori G27; NCBI TaxID: 563041; scientific name: Helicobacter pylori PMSS1; NCBI TaxID: 1431450;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-04 12:18:51ID requested
12025-04-08 05:05:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: in-vivo mouse infection, full proteome,Helicobacter pylori, secretome, LC-MS/MS
Contact List
Stephan Axel Sieber
contact affiliationTechnical University of Munich (TUM), TUM School of Natural Sciences, Department Biosciences, Chair of Organic Chemistry II, Center for Functional Protein Assemblies (CPA), Ernst-Otto-Fischer Str. 8, Garching 85748, Germany
contact emailstephan.sieber@tum.de
lab head
Nina Bach
contact affiliationTU München
contact emailnina.bach@tum.de
dataset submitter
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Dataset FTP location
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