PXD050332

PXD050332 is an original dataset announced via ProteomeXchange.

Title	Global Proteomic Analysis of Ovarian Clear Cell Carcinoma Cells Lacking ARID1A Reveals Upregulated Mitochondrial Electron Transport Chain Activity
Description	Ovarian clear cell carcinoma (OCCC) is the most lethal gynecological cancer. It is characterized by somatic inactivating mutations of ARID1A, a component of the SWI/SNF chromatin-remodeling complex, occurring in up to 70% of patients. Patients with these mutations in their tumors have considerably poorer outcomes compared to those without such mutations. ARID1A-deficient cells have been shown to have a higher dependence on mitochondrial respiration, suggesting that targeting mitochondrial respiration is a promising approach to eliminating ARID1A-deficient cancer cells. Here we generated and characterized OCCC-derived ARID1A wild type and knock-out cell lines. Our proteomic data provide evidence of the increased relative abundance of mETC proteins in the ARID1A knock-out OCCC cells. Taken together, our data provides a rationale for identifying therapeutic vulnerabilities within the mETC in the context of treating ARID1A-deficient OCCC.
HostingRepository	PRIDE
AnnounceDate	2025-07-07
AnnouncementXML	Submission_2025-07-06_16:14:10.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefani Thomas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-04 11:48:24	ID requested
⏵ 1	2025-07-06 16:14:11	announced

Perez JM, Ryu J, Khan H, Shetty M, Parker E, D'Arcy P, Zhu S, Bazzaro M, Thomas SN, Proteomic Analysis of ARID1A-Deficient Ovarian Clear Cell Carcinoma Cells Reveals Differential Mitochondria ETC Subunit Abundances and Targetable Mitochondrial Pathways. Int J Mol Sci, 26(12):(2025) [pubmed]

10.3390/ijms26125466;

submitter keyword: mitochondrial electron transport chain, mitochondria,ARID1A, ovarian clear cell carcinoma

Stefani N. Thomas
contact affiliation	Department of Laboratory Medicine and Pathology University of Minnesota School of Medicine 420 Delaware Street SE, MMC 609 Minneapolis, MN
contact email	stefanit@umn.edu
lab head
Stefani Thomas
contact affiliation	University of Minnesota
contact email	stefanit@umn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050332
     1. Label: PRIDE project
     2. Name: Global Proteomic Analysis of Ovarian Clear Cell Carcinoma Cells Lacking ARID1A Reveals Upregulated Mitochondrial Electron Transport Chain Activity