PXD050329 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An automated workflow based on data independent acquisition for practical and high-throughput personalized assay development and minimal residual disease monitoring in multiple myeloma patients |
Description | Minimal residual disease (MRD) status in multiple myeloma (MM) is an important prognostic biomarker. Personalized blood-based targeted mass spectrometry (MS-MRD) detecting M-proteins was shown to provide a sensitive and minimally invasive alternative to MRD assessment in bone marrow. However, MS-MRD still comprises manual steps that hamper upscaling of sample size. Here, we introduce a proof-of-concept for a novel workflow using dia-PASEF technology and automated data processing.Using automated data processing of PASEF DDA and dia-PASEF measurements, we developed a workflow that identified unique targets from MM patient sera and personalized protein sequence databases. We generated patient-specific libraries linked to dia-PASEF methods and subsequently quantitated and reported M-protein concentrations in follow-up samples from MM patients. Assay performance of prm-PASEF and dia-PASEF workflows were compared and we tested mixing patient intake sera for multiplexed target identification and selection. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-10 |
AnnouncementXML | Submission_2025-06-10_09:50:07.276.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hans Wessels |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-04 08:50:19 | ID requested | |
⏵ 1 | 2025-06-10 09:50:07 | announced | |
Publication List
Keyword List
submitter keyword: multiple myeloma, dia-PASEF, minimal residual disease, multiplexing, M-protein |
Contact List
Hans Wessels |
contact affiliation | Translational Metabolic Laboratory, Department of Human Genetics, Radboudumc, Nijmegen, The Netherlands |
contact email | hans.wessels@radboudumc.nl |
lab head | |
Hans Wessels |
contact affiliation | Radboudumc |
contact email | hans.wessels@radboudumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD050329 |
PRIDE project URI |
Repository Record List
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- PRIDE
- PXD050329
- Label: PRIDE project
- Name: An automated workflow based on data independent acquisition for practical and high-throughput personalized assay development and minimal residual disease monitoring in multiple myeloma patients