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PXD050329

PXD050329 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn automated workflow based on data independent acquisition for practical and high-throughput personalized assay development and minimal residual disease monitoring in multiple myeloma patients
DescriptionMinimal residual disease (MRD) status in multiple myeloma (MM) is an important prognostic biomarker. Personalized blood-based targeted mass spectrometry (MS-MRD) detecting M-proteins was shown to provide a sensitive and minimally invasive alternative to MRD assessment in bone marrow. However, MS-MRD still comprises manual steps that hamper upscaling of sample size. Here, we introduce a proof-of-concept for a novel workflow using dia-PASEF technology and automated data processing.Using automated data processing of PASEF DDA and dia-PASEF measurements, we developed a workflow that identified unique targets from MM patient sera and personalized protein sequence databases. We generated patient-specific libraries linked to dia-PASEF methods and subsequently quantitated and reported M-protein concentrations in follow-up samples from MM patients. Assay performance of prm-PASEF and dia-PASEF workflows were compared and we tested mixing patient intake sera for multiplexed target identification and selection.
HostingRepositoryPRIDE
AnnounceDate2025-06-10
AnnouncementXMLSubmission_2025-06-10_09:50:07.276.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHans Wessels
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-04 08:50:19ID requested
12025-06-10 09:50:07announced
Publication List
10.1515/CCLM-2024-0306;
Keyword List
submitter keyword: multiple myeloma, dia-PASEF, minimal residual disease, multiplexing, M-protein
Contact List
Hans Wessels
contact affiliationTranslational Metabolic Laboratory, Department of Human Genetics, Radboudumc, Nijmegen, The Netherlands
contact emailhans.wessels@radboudumc.nl
lab head
Hans Wessels
contact affiliationRadboudumc
contact emailhans.wessels@radboudumc.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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