PXD050326

PXD050326 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CryoEM architecture of a near-native stretch-sensitive membrane microdomain
Description	Eisosomes, stress-sensitive membrane compartments in yeast, are scaffolded by the BAR-domain proteins Pil1 and Lsp1. We isolated near-native eisosomes as helical filaments of Pil1/Lsp1 lattice bound to plasma membrane lipids and solved their structure by helical reconstruction. From the same near-native eisosome samples, we performed mass spectroscopy to determine the protein content, and the abundance ratio of Pil1/Lsp1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:04.862.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nesli Ece Sen
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-04 07:48:45	ID requested
1	2024-08-23 05:05:37	announced
2	2024-08-23 06:01:55	announced	2024-08-23: Updated project metadata.
⏵ 3	2024-10-22 06:56:05	announced	2024-10-22: Updated project metadata.

Publication List

Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gaji, ć J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R, Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain. Nature, 632(8025):664-671(2024) [pubmed]
10.1038/s41586-024-07720-6;
10.1038/S41586-024-07720-6;

Kefauver JM, Hakala M, Zou L, Alba J, Espadas J, Tettamanti MG, Gaji, ć J, Gabus C, Campomanes P, Estrozi LF, Sen NE, Vanni S, Roux A, Desfosses A, Loewith R, Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain. Nature, 632(8025):664-671(2024) [pubmed]

10.1038/s41586-024-07720-6;

10.1038/S41586-024-07720-6;

Keyword List

submitter keyword: Membrane microdomain, eisosome, Pil1, Lsp1

submitter keyword: Membrane microdomain, eisosome, Pil1, Lsp1

Contact List

Prof. Robbie Loewith
contact affiliation	Department of Molecular and Cellular Biology Faculty of Sciences III University of Geneva
contact email	robbie.loewith@unige.ch
lab head
Nesli Ece Sen
contact affiliation	University of Geneva
contact email	nesli.sen@unige.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050326
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050326

PRIDE project URI

Repository Record List

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