PXD050324

PXD050324 is an original dataset announced via ProteomeXchange.

Title	The Hansenula polymorpha Mitochondrial Carrier Family protein Mir1 is dually localized at peroxisomes and mitochondria
Description	Peroxisomes are ubiquitous cell organelles involved in various metabolic pathways. In order to properly function, several cofactors, substrates and products of peroxisomal enzymes need to pass the organellar membrane. So far only a few transporter proteins have been identified. We analysed peroxisomal membrane fractions purified from the yeast Hansenula polymorpha by untargeted label-free quantitation mass spectrometry. As expected, several known peroxisome-associated proteins were enriched in the peroxisomal membrane fraction. In addition, several other proteins were enriched, including mitochondrial transport proteins. Localization studies revealed that one of them, the mitochondrial phosphate carrier Mir1, has a dual localization on mitochondria and peroxisomes. To better understand the molecular mechanisms of dual sorting, we localized Mir1 in cells lacking Pex3 or Pex19, two peroxins that play a role in targeting of peroxisomal membrane proteins. In these cells Mir1 only localized to mitochondria, indicating that Pex3 and Pex19 are required to sort Mir1 to peroxisomes. Analysis of the localization of truncated versions of Mir1 in wild-type H. polymorpha cells revealed that most of them localized to mitochondria, but only one, consisting of the transmembrane domains 3-6, was peroxisomal. Peroxisomal localization of this construct was lost in a MIR1 deletion strain, indicating that full length Mir1 was required for the localization of the truncated protein to peroxisomes. Our data suggest that only full length Mir1 sorts to peroxisomes, while Mir1 contains multiple regions with mitochondrial sorting information.
HostingRepository	PRIDE
AnnounceDate	2024-05-10
AnnouncementXML	Submission_2024-05-10_02:06:05.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justina Clarinda Wolters
SpeciesList	scientific name: Hansenula polymorpha vector pAM619; NCBI TaxID: 1538089;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-04 07:14:07	ID requested
⏵ 1	2024-05-10 02:06:05	announced

Pedersen MP, Wolters JC, de Boer R, Krikken AM, van der Klei IJ, The Hansenula polymorpha mitochondrial carrier family protein Mir1 is dually localized at peroxisomes and mitochondria. Biochim Biophys Acta Mol Cell Res, 1871(5):119742(2024) [pubmed]

10.1016/j.bbamcr.2024.119742;

submitter keyword: mitochondrial carrier family,Peroxisomes, mitochondria, transporter proteins, Mir1, dual localization

Justina Clarinda
contact affiliation	Laboratory of Pediatrics, Section Systems Medicine of Metabolism and Signaling, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
contact email	j.c.wolters@umcg.nl
lab head
Justina Clarinda Wolters
contact affiliation	UMCG
contact email	j.c.wolters@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD050324

PRIDE project URI

• PRIDE
  1. PXD050324
     1. Label: PRIDE project
     2. Name: The Hansenula polymorpha Mitochondrial Carrier Family protein Mir1 is dually localized at peroxisomes and mitochondria