PXD050321

PXD050321 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial ribosomal protein MRPS15 Is a component of cytosolic ribosomes and regulates translation in stressed cardiomyocytes
Description	Regulation of mRNA translation is a crucial step in controlling gene expression in stressed cells, impacting many pathologies, including heart ischemia. In recent years, ribosome heterogeneity has emerged as a key control mechanism driving the translation of subsets of mRNAs. In this study, we investigated variations in ribosome composition in human cardiomyocytes subjected to endoplasmic reticulum stress induced by tunicamycin treatment. Our findings demonstrate that this stress inhibits global translation in cardiomyocytes while activating IRES-dependent translation. Analysis of translating ribosome composition in stressed and unstressed cardiomyocytes was conducted using mass spectrometry. We observed no significant changes in ribosomal protein composition, but several mitochondrial ribosomal proteins (MRPs) were identified in cytosolic polysomes, showing drastic variations between stressed and unstressed cells. The most notable increase in polysomes of stressed cells was observed in MRPS15. Its interaction with ribosomal proteins was confirmed by proximity ligation assay (PLA) and immunoprecipitation, suggesting its intrinsic role as a ribosomal component during stress. Knock-down or overexpression experiments of MRPS15 revealed its role as an activator of IRES-dependent translation. Furthermore, polysome profiling after immunoprecipitation with anti-MRPS15 antibody revealed that the “MRPS15 ribosome” is specialized in translating mRNAs involved in the unfolded protein response.
HostingRepository	PRIDE
AnnounceDate	2024-06-23
AnnouncementXML	Submission_2024-06-23_01:38:07.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carine Froment
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; methylated residue; phosphorylated residue; mono N-acetylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-04 05:51:06	ID requested
⏵ 1	2024-06-23 01:38:08	announced

David F, Roussel E, Froment C, Draia-Nicolau T, Pujol F, Burlet-Schiltz O, Henras AK, Lacazette E, Morfoisse F, Tatin F, Diaz JJ, Catez F, Garmy-Susini B, Prats AC, Mitochondrial Ribosomal Protein MRPS15 Is a Component of Cytosolic Ribosomes and Regulates Translation in Stressed Cardiomyocytes. Int J Mol Sci, 25(6):(2024) [pubmed]

10.3390/ijms25063250;

submitter keyword: Ribosome heterogeneity

Mitochondrial ribosomal protein

Translational control

IRES

Endoplasmic reticulum stress

Cardiomyocyte

Proteomics

Anne-Catherine Prats
contact affiliation	UMR 1297-I2MC, Inserm, Université de Toulouse, UPS , 31000Toulouse, France
contact email	anne-catherine.prats@inserm.fr
lab head
Carine Froment
contact affiliation	IPBS-CNRS
contact email	carine.froment@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050321
     1. Label: PRIDE project
     2. Name: Mitochondrial ribosomal protein MRPS15 Is a component of cytosolic ribosomes and regulates translation in stressed cardiomyocytes