PXD050305

PXD050305 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of bias gas flow strategy at birth
Description	This study aimed to use a lamb model of preterm birth to investigate the impact of different bias gas flow strategies, applied during positive pressure ventilation, on lung injury development. Preterm lambs were ventilated for a total of 90 minutes. A group of lambs was euthanised at birth to act as an unventilated control group. At post-mortem lung tissue was sampled for proteomic analysis. Lung tissue samples were analysed by mass spectrometry-based proteomics using a TMT-11plex labelling approach, followed by nano-liquid chromatography coupled to an Eclipse Orbitrap mass spectrometer in a data-dependent acquisition mode.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:34:06.550.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Monique Fatmous
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-03 22:52:53	ID requested
⏵ 1	2025-05-06 13:34:07	announced

Tingay DG, Fatmous M, Kenna K, Chapman J, Douglas E, Sett A, Poh QH, Dahm SI, Quach TK, Sourial M, Fang H, Greening DW, Pereira-Fantini PM, Speed of lung inflation at birth influences the initiation of lung injury in preterm lambs. JCI Insight, 9(18):(2024) [pubmed]

10.1172/jci.insight.181228;

submitter keyword: lung proteomics,preterm lung injury, bias gas flow

David Tingay
contact affiliation	1Neonatal Research, Murdoch Children’s Research Institute, Parkville, Australia 2Department of Paediatrics, University of Melbourne, Melbourne, Australia
contact email	david.tingay@mcri.edu.au
lab head
Monique Fatmous
contact affiliation	Murdoch Children's Research Institute
contact email	monique.fatmous@mcri.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050305

PRIDE project URI

• PRIDE
  1. PXD050305
     1. Label: PRIDE project
     2. Name: Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of bias gas flow strategy at birth