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PXD050301

PXD050301 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomics analysis of root and nodule mitochondria of soybean
DescriptionLegumes perform symbiotic nitrogen fixation through rhizobial bacteroids housed in specialised root nodules. The biochemical process is energy‐intensive and consumes a huge carbon source to generate sufficient reducing power. To maintain the symbiosis, malate is supplied by legume nodules to bacteroids as their major carbon and energy source in return for ammonium ions and nitrogenous compounds. To sustain the carbon supply to bacteroids, nodule cells undergo drastic reorganisation of carbon metabolism. Here, a comprehensive quantitative comparison of the mitochondrial proteomes between root nodules and uninoculated roots was performed using data‐independent acquisition proteomics, revealing the modulations in nodule mitochondrial proteins and pathways in response to carbon reallocation. Corroborated our findings with that from the literature, we believe nodules preferably allocate cytosolic phosphoenolpyruvates towards malate synthesis in lieu of pyruvate synthesis, and nodule mitochondria prefer malate over pyruvate as the primary source of NADH for ATP production. Moreover, the differential regulation of respiratory chain‐associated proteins suggests that nodule mitochondria could enhance the efficiencies of complexes I and IV for ATP synthesis. This study highlighted a quantitative proteomic view of the mitochondrial adaptation in soybean nodules.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:50:02.272.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWai Ching SIN
SpeciesList scientific name: Glycine max; NCBI TaxID: 3847;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-03 21:57:36ID requested
12024-07-15 19:56:40announced
22024-10-22 06:50:02announced2024-10-22: Updated project metadata.
Publication List
10.1111/PCE.15026;
Keyword List
submitter keyword: nitrogen fixation,Data-independent acquisition (DIA) proteomics, electron transport chain, Glycine max, mitochondria, malate, nodule, legume, soybean
Contact List
Boon Leong Lim
contact affiliationSchool of Biological Sciences, University of Hong Kong; Pokfulam, Hong Kong, China
contact emailbllim@hku.hk
lab head
Wai Ching SIN
contact affiliationSchool of Life Sciences, The Chinese University of Hong Kong
contact emailwaichingsin@cuhk.edu.hk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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