PXD050297

PXD050297 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HYPer-SPM: Ultra Efficient Peptide Purification by a Pipette-tip Microcolumn Enabled by Hydrophobic Microparticles Hybridyzed Sponge-like Monolithic Carrier
Description	Peptide purification techniques with minimal loss play a crucial role in enhancing sensitivity and coverage in mass spectrometry (MS)-based proteomics. Here, we developed a pipette tip-based microcolumn with hydrophobic particles embedded in a sponge-like monolithic carrier, named HYPer-SPM, achieving highly efficient peptide recovery in peptide purification by solid-phase extraction (SPE). HYPer-SPM was synthesized by thermal mixing thermoplastic resins of ethylene-vinyl acetate copolymer (EVA) with styrene-divinylbenzene copolymer (St-DVB) particles. The column packing process of HYPer-SPM was easy and reproducible due to its sponge-like flexibility, serving simultaneously as a column material and frit in a pipette tip. Peptide samples were comprehensively retained on the hydrophobic surface of HYPer-SPM, while thermally melted EVA resins effectively prevented the irreversible adsorption of peptides into the mesopores of St-DVB particles by filling them. The unique morphology of HYPer-SPM resulted in the identification of more than double the number of peptides compared to the standard StageTip method. Furthermore, the commonly identified peptide intensities were consistently higher in HYPer-SPM over the entire retention time range. The highly efficient peptide recovery of HYPer-SPM was especially emphasized for hydrophobic and longer peptides. Our HYPer-SPM holds great promise as a peptide purification method in ultra-sensitive proteomics, finding potential applications in clinical proteomics, single-cell proteomics, and deep visual proteomics by integrating other sample preparation methods.
HostingRepository	jPOST
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-03_07:00:05.048.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shunsuke Tanaka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	instrument; instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-03 19:55:45	ID requested
⏵ 1	2025-03-03 07:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Peptide purification, Bottom-up proteomics, StageTip, Sponge-like polymer

submitter keyword: Peptide purification, Bottom-up proteomics, StageTip, Sponge-like polymer

Contact List

Yasushi Ishihama
lab head
Shunsuke Tanaka
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002969/