PXD050296

PXD050296 is an original dataset announced via ProteomeXchange.

Title	Bacteroides thetaiotaomicron grown on different sources of dietary protein and glucose
Description	We previously observed in mice that Bacteroides thetaiotaomicron (B. theta) significantly increased in abundance in the gut microbiome of mice when mice were fed egg and yeast dietary protein sources. We also observed that B. theta was expressing proteins previously connected to growth on mucin glycans when mice were fed an egg-white diet. To confirm that the bacterium were actually responding to the protein sources, we grew it in vitro using a defined medium, where the sole carbon source was dietary protein, mucin, or glucose. Our controls were glucose and mucin, and our experimental protein sources were soy protein, egg-white protein, and Torula yeast protein. We grew four B. theta cultures per carbon source statically at 37°C in a Coy anaerobic chamber (2.5 % H2 /10 % CO2 /88.5 % N2) in minimal medium (100 mM KH2PO4, 8.5 mM [NH2]4SO4, 15 mM NaCl, 5.8 μM vitamin K3, 1.44 μM FeSO4⋅7H2O, 1 mM MgCl2, 1.9 μM hematin, 0.2 mM L-histidine, 3.69 nM vitamin B12, 208 μM L-cysteine, and 7.2 μM CaCl2⋅2H2O) with one of the above mentioned nutrients added at 0.5% (wt/v) concentration. In order to aid the solubilization of the dietary proteins, we pre-prepared the proteins in 200 mM NaOH at 37°C for four days, the glucose control was also dissolved in 200 mM NaOH. After 8 hours, we enumerated CFUs to confirm growth, and we pelleted cells by centrifuging at 4,000 g for 10 minutes. We then removed the supernatant and froze the pellets at -80°C within 30 minutes.
HostingRepository	PRIDE
AnnounceDate	2024-12-18
AnnouncementXML	Submission_2024-12-18_12:46:19.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	J. Alfredo Blakeley Ruiz
SpeciesList	scientific name: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482); NCBI TaxID: 226186;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-03 19:47:53	ID requested
⏵ 1	2024-12-18 12:46:20	announced

Blakeley-Ruiz JA, Bartlett A, McMillan AS, Awan A, Vanhoy Walsh M, Meyerhoffer AK, Vintila S, Maier JL, Richie T, Theriot CM, Kleiner M, Dietary protein source alters gut microbiota composition and function. bioRxiv, ():(2025) [pubmed]

10.1101/2024.04.04.588169;

submitter keyword: Protein Media, Bacteroides thetaiotaomicron,Gut bacteria

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
J. Alfredo Blakeley Ruiz
contact affiliation	North Carolina State University, Raliegh, NC
contact email	jablakel@ncsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050296
     1. Label: PRIDE project
     2. Name: Bacteroides thetaiotaomicron grown on different sources of dietary protein and glucose