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PXD050291

PXD050291 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiple LC-MS platforms analysis under standardized quality control system of urinary proteomics for precision medicine studies
DescriptionIn this study, we carried out a comprehensive evaluation of multiple LC-MS platforms in urinary proteomics including 720 proteome measurements. A series of interrelated studies was designed to assess the intra- and inter-platform consistency and reproducibility. Firstly, a uniformly prepared urine sample is distributed to 20 LC-MS platforms, where a total of 160 data are generated to analyze the consistency and reproducibility across different LC-MS platforms. Secondly, benchmarking samples, which consist of tryptic digests of human, yeast, and E. coli proteins mixed in defined proportions, are generated to mimic differential expressed biological samples and provide proof of the robustness and reproducibility of the different LC-MS platforms. Finally, the above methodologies are applied to actual large cohort clinical CRC urinary proteome datasets derived from 3 LC-MS platforms to further demonstrate the performance of urinary proteomics cohorts from multi-platform in biomarker discovery.
HostingRepositoryiProX
AnnounceDate2024-03-01
AnnouncementXMLSubmission_2024-03-03_19:33:12.268.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiang Liu
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Q Exactive HF; timsTOF Pro; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-03 19:32:30ID requested
12024-03-03 19:33:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Urinary proteomics, Multiple LC-MS platforms
Contact List
Wei Sun
contact affiliationInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China
contact emailsunwei@ibms.pumc.edu.cn
lab head
Xiang Liu
contact affiliationBeihang University; Peking Union Medical College
contact emaillx940527@126.com
dataset submitter
Full Dataset Link List
iProX dataset URI