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PXD050261

PXD050261 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling Cullin4-E3 ligases interactomes and their rewiring in influenza A virus infection
DescriptionUnderstanding the integrated regulation of cellular processes during viral infection is crucial for developing host-targeted approaches. We have previously reported that an optimal in-vitro infection by influenza A (IAV) requires three components of Cullin 4-RING E3 ubiquitin ligases (CRL4), namely the DDB1 adaptor and two Substrate Recognition Factors (SRF), DCAF11 and DCAF12L1, which mediate non-degradative poly-ubiquitination of the PB2 subunit of the viral polymerase. However, the impact of IAV infection on the CRL4 interactome remains elusive. Here, using Affinity Purification coupled with Mass Spectrometry (AP-MS) approaches, we identified cellular proteins interacting with these CRL4 components in IAV-infected and non-infected contexts. IAV infection induces significant modulations in protein interactions, resulting in a global loss of DDB1 and DCAF11 interactions, and an increase of DCAF12L1-associated proteins. The distinct rewiring of CRL4’s associations upon infection impacted cellular proteins involved in protein folding, ubiquitination, translation, splicing, and stress responses. Using a split-nanoluciferase-based assay, we identified direct partners of CRL4 components, representing potential substrates or regulators of CRL4 complexes. Our findings unravel the dynamic remodeling of the proteomic landscape of CRL4’s E3 ubiquitin ligases during IAV infection, likely involved in shaping a cellular environment conducive to viral replication and offers potential for the exploration of future host-targeted antiviral therapeutic strategies.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_13:28:32.120.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThibaut Douché
SpeciesList scientific name: Influenza A virus (A/WSN/1933(H1N1)); NCBI TaxID: 382835; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-01 02:01:30ID requested
12025-05-06 13:28:33announced
Publication List
10.1016/j.mcpro.2024.100856;
Keyword List
submitter keyword: Human, AP-MS, Q Exactive Plus, influenza virus
Contact List
Caroline Demeret
contact affiliationInteractomics, RNA and Immunity Laboratory, Institut Pasteur, 75015 Paris, France
contact emailcaroline.demeret@pasteur.fr
lab head
Thibaut Douché
contact affiliationInstitut Pasteur
contact emailthibaut.douche@pasteur.fr
dataset submitter
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