PXD050250

PXD050250 is an original dataset announced via ProteomeXchange.

Title	Murine Mammary fat pad on high fat diet
Description	Younger age and obesity increase the incidence and rates of metastasis of triple-negative breast cancer (TNBC), an aggressive subtype of breast cancer. The tissue microenvironment, specifically the extracellular matrix (ECM), is known to promote tumor invasion and metastasis. We sought to characterize the effect of both age and obesity on the ECM of mammary fat pads. We used a diet-induced obesity (DIO) model where 10-week-old female mice were fed a high-fat diet (HFD) for 16 weeks or a control chow diet (CD) where time points were every 4 weeks to monitor age and obesity HFD progression. We isolated the mammary fat pads to characterize the ECM at each time point. Utilizing proteomics, we found that the early stages of obesity were sufficient to induce distinct differences in the ECM composition of mammary fat pads that promote TNBC cell invasion. ECM proteins previously implicated in driving TNBC invasion Collagen IV and Collagen VI, were enriched with weight gain. Together these data implicate ECM changes in the primary tumor microenvironment as mechanisms by which age and obesity contribute to breast cancer progression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:54.837.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sydney Conner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	(15)N isotope tagged reagent
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-29 15:57:31	ID requested
1	2024-06-06 11:43:21	announced
⏵ 2	2024-10-22 06:43:55	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: mammary fat pad, obesity, High fat diet

Madeleine Oudin
contact affiliation	Tufts University
contact email	madeleine.oudin@tufts.edu
lab head
Sydney Conner
contact affiliation	Tufts University
contact email	sydney.conner@tufts.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050250
     1. Label: PRIDE project
     2. Name: Murine Mammary fat pad on high fat diet