PXD050249

PXD050249 is an original dataset announced via ProteomeXchange.

Title	Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis
Description	In Saccharomyces cerevisiae, most ribosomal proteins are produced from duplicated genes. These nearly identical protein pairs are expressed at varying levels, with one ‘major paralog’ usually predominating. The minor paralog is highly transcribed but held in check through reduced intron removal, but the mechanism and purpose of this copy-specific repression remains unclear. In this study, we searched for proteins that achieve copy-specific expression by acting through the intron of the minor paralog of the model duplicated small ribosomal subunit protein S9 genes. By mass spectrometry and gene deletion we demonstrate that the transcription factors Rim101 and Taf14 bind to the intron and inhibit the splicing of the minor RPS9 paralog. RPS9A is then specifically de-repressed during meiosis to ensure optimal expression of meiotic genes and efficient sporulation. Our results reveal a new regulatory paradigm where transcriptional factors can also modulate splicing rates to optimize the expression of duplicated ribosomal protein genes.
HostingRepository	PRIDE
AnnounceDate	2024-12-20
AnnouncementXML	Submission_2024-12-20_14:57:08.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathieu Catala
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-29 15:14:34	ID requested
⏵ 1	2024-12-20 14:57:08	announced

Dataset with its publication pending

submitter keyword: splicing, ribosomal proteins,yeast

Sherif Abou Elela
contact affiliation	Département de microbiologie et infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke
contact email	sherif.abou.elela@usherbrooke.ca
lab head
Mathieu Catala
contact affiliation	Universite de Sherbrooke
contact email	mathieu.catala@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD050249

PRIDE project URI

• PRIDE
  1. PXD050249
     1. Label: PRIDE project
     2. Name: Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis