PXD050243

PXD050243 is an original dataset announced via ProteomeXchange.

Title	Quantification of histone H1 subtypes using targeted proteomics
Description	Histone H1 is involved in the regulation of chromatin structure. Human somatic cells express up to seven subtypes: H1.0, H1.1-H15, and H1X. The variability in the proportions of somatic H1s (H1 complement) is one evidence supporting their functional specificity. Alterations in the protein levels of different H1 subtypes have been observed in cancer, suggesting their potential as biomarkers and that they might play a role in disease development. These reasons led us to develop a mass spectrometry based (MS) parallel reaction monitoring (PRM) assay suitable for the quantification of H1 subtypes. Our PRM method is based on the quantification of unique peptides for each subtype, providing high specificity. Evaluation of the PRM performance on three human cell lines showed high reproducibility and sensitivity. Quantification values agreed with the electrophoretic and Western blot, indicating the accuracy of the results. We used PRM to characterize the H1 complement in peripheral blood samples of healthy individuals, finding that the more abundant subtypes were H1.4 and H1.5. Analysis by PRM of samples from chronic myeloid leukemia patients showed that higher levels of H1 were associated with imatinibresistance, suggesting its potential as a predictive biomarker. Non-responder patients had lower proportions of H1.0, H1.1, and H1X than responders, hinting that their alteration could be involved in the acquisition of resistance.
HostingRepository	PRIDE
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2024-11-05_03:09:14.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Vilaseca
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-29 08:23:39	ID requested
⏵ 1	2024-11-05 03:09:15	announced

10.3390/BIOM14101221;

submitter keyword: imatinib resistance, chronic myeloid leukemia, functional differentiation, cancer biomarker, parallel reaction monitoring,Histone H1

Marta Vilaseca
contact affiliation	Mass Spectrometry & Proteomics Core Facility (MSPCF) Institute for Research in Biomedicine (IRB Barcelona) Barcelona Institute of Science and Technology (BIST) C/ Baldiri Reixac, 10-12 08028 Barcelona - Spain.
contact email	masspe@irbbarcelona.org
lab head
Marta Vilaseca
contact affiliation	Mass Spectrometry & Proteomics Core Facility (MSPCF) Institute for Research in Biomedicine (IRB Barcelona) Barcelona Institute of Science and Technology (BIST) C/ Baldiri Reixac, 10-12 08028 Barcelona - Spain
contact email	masspe@irbbarcelona.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD050243

PRIDE project URI

• PRIDE
  1. PXD050243
     1. Label: PRIDE project
     2. Name: Quantification of histone H1 subtypes using targeted proteomics