PXD050242

PXD050242 is an original dataset announced via ProteomeXchange.

Title	Proteolytic activity of surface exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile
Description	To survive in the host, pathogenic bacteria need to be able to react to the unfavourable conditions that they encounter, like low pH, elevated temperatures, antimicrobial peptides and many more. These conditions may lead to unfolding of envelope proteins and this may be lethal. One of the mechanisms through which bacteria are able to survive these conditions is through the protease/foldase activity of the high temperature requirement A (HtrA) protein. The gut pathogen Clostridioides difficile encodes one HtrA homolog that is predicted to contain a membrane anchor and a single PDZ domain. The function of HtrA in C. difficile is hitherto unknown but previous work has shown that an insertional mutant of htrA displayed elevated toxin levels, less sporulation and decreased binding to target cells. Here, we show that HtrA is membrane associated and localized on the surface of C. difficile and characterize the requirements for proteolytic activity of recombinant soluble HtrA. In addition, we show that the level of HtrA in the bacteria heavily depends on the proteolytic activity. Finally, we show that proteolytic activity of HtrA is required for survival under acidic conditions.
HostingRepository	PRIDE
AnnounceDate	2025-11-11
AnnouncementXML	Submission_2025-11-11_08:44:54.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bart Claushuis
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: NEWT:272563;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-29 08:10:49	ID requested
⏵ 1	2025-11-11 08:44:55	announced

Corver J, Claushuis B, Shamorkina TM, de Ru AH, van Leeuwen MM, Hensbergen PJ, Smits WK, Proteolytic activity of surface-exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile. Mol Microbiol, 122(4):413-428(2024) [pubmed]

10.1111/mmi.15300;

submitter keyword: acid stress, Clostridioides difficile, PDZ domain, surface proteins, dimethyl labeling,HtrA, gene regulation, Protease, LC-MS/MS

P. A. van Veelen
contact affiliation	Center for Proteomics and Metabolomics, Leiden University Medical Center, The Netherlands
contact email	p.a.van_veelen@lumc.nl
lab head
Bart Claushuis
contact affiliation	Leiden University Medical Center
contact email	b.claushuis@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD050242

PRIDE project URI

• PRIDE
  1. PXD050242
     1. Label: PRIDE project
     2. Name: Proteolytic activity of surface exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile