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PXD050237

PXD050237 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffect of aberrant PlGF on endometrial stromal cell actin dynamics : implications in pre-eclampsia.
DescriptionHigh levels of placental growth factor (PlGF) are pathological, however its function in endometrial cells remains to be investigated. Cell proliferation and motility requires actin reorganization, which is under the control of various signalling pathways including Rac1 and PAK1 proteins. In this study, we explored whether PlGF induces change in endometrial mechanics by modifying actin cytoskeleton thus contributing to cell stiffness at the maternal interface. To this end, human endometrial stromal cells (EnSCs) were treated with 20 ng/mL for 6 days with PlGF and its influence on cellular mechanics was investigated with atomic force microscopy (AFM), electrical impedance spectroscopy and proteomics methods. Proteomic analysis shows PlGF upregulated RhoGTPases activating proteins and extracellular matrix organization associated proteins. Rac1 and PAK1 transcript levels, activity and actin polymerization were significantly increased with in vitro PlGF treatment. AFM further revealed an increase in cell stiffness with PlGF. The PlGF effect on actin polymerization was inhibited with siRNA- Rac1, PAK1 and WAVE2. PlGF induced cell stiffness was pharmacologically reversed with pravastatin, resulting in improved trophoblast cell invasion as measured using electrical impedance spectroscopy. Thus, PlGF treatment leads to enhanced Rac1 and PAK1 levels, leading to an increase in cell stiffness, impairing trophoblast invasion. Taken together, aberrant PlGF levels can contribute to an altered pre-pregnancy maternal microenvironment and offers a coherent and unifying explanation for the pathological changes observed in pre-eclampsia (PE) conditions.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:46:43.959.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAna Velic
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-29 04:30:44ID requested
12024-06-23 01:19:49announced
22024-10-22 06:46:44announced2024-10-22: Updated project metadata.
Publication List
Raja Xavier JP, Rianna C, Hellwich E, Nikolou I, Lankapalli AK, Brucker SY, Singh Y, Lang F, Sch, ä, ffer TE, Salker MS, Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia. Commun Biol, 7(1):530(2024) [pubmed]
10.1038/s42003-024-06220-7;
Keyword List
submitter keyword: actin polymerization, cell stiffness,Endometrial cells, angiogenesis, pre-eclampsia.
Contact List
Madhuri Salker
contact affiliationForschungsinstitut für Frauengesundheit Universitäts- Frauenklinik Uni Tübingen Germany
contact emailMadhuri.Salker@med.uni-tuebingen.de
lab head
Ana Velic
contact affiliationUniversity of Tuebingen
contact emaila.velic@uni-tuebingen.de
dataset submitter
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