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PXD050227

PXD050227 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLong term adaptation of lymphoma cells to hypoxia
DescriptionThis study explores the long-term adaptation mechanisms of lymphoma cells subjected to hypoxic conditions, with an emphasis on the HBL2 and Ramos cell lines under normoxia and 1% O2 environments. The research methodology encompassed lysing cells using a buffer containing sodium deoxycholate and TEAB, followed by protein quantification via a BCA assay. Subsequent steps involved protein digestion with trypsin, labelling with TMTpro™ 16plex Label Reagent Set for quantitative analysis, peptide fractionation, and LC-MS/MS analysis. The analytical process was supported by Proteome Discoverer 2.4 for protein identification and quantification. Experimental groups were categorized into "HBL2 normoxia," "HBL2 1% O2 adaptation," "Ramos normoxia," and "Ramos 1% O2 adaptation," conducted in triplicates to ensure reliability. This meticulous approach facilitated the delineation of unique proteomic landscapes indicative of hypoxia adaptation, unveiling cellular strategies employed by lymphoma cells to navigate low oxygen conditions. The findings advance our comprehension of how hypoxia influences cancer progression and potentially opens new avenues for targeting hypoxic niches within tumors.
HostingRepositoryPRIDE
AnnounceDate2025-02-27
AnnouncementXMLSubmission_2025-02-27_02:01:00.489.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatěj Běhounek
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-28 15:05:42ID requested
12025-02-27 02:01:01announced
Publication List
Daumova L, Manakov D, Petrak J, Sovilj D, Behounek M, Andera L, Vit O, Souckova O, Havranek O, Dolnikova A, Renesova N, Tuskova L, Winkowska L, Bettazova N, Kupcova K, Kalbacova MH, Sikorova M, Trneny M, Klener P, Long-term adaptation of lymphoma cell lines to hypoxia is mediated by diverse molecular mechanisms that are targetable with specific inhibitors. Cell Death Discov, 11(1):65(2025) [pubmed]
10.1038/s41420-025-02341-y;
Keyword List
submitter keyword: Lymphoma cells (HBL2, TMT Proteomic analysis,Hypoxia adaptation, Ramos), Cellular mechanisms
Contact List
prof. MUDr. Pavel Klener, Ph.D.
contact affiliationInstitute of Pathological Physiology First Faculty of Medicine, Charles University
contact emailpavel.klener@gmail.com
lab head
Matěj Běhounek
contact affiliationBIOCEV, First Faculty of Medicine, Charles University, Prague, Czech Republic
contact emailmatej.behounek@lf1.cuni.cz
dataset submitter
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Dataset FTP location
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