PXD050227 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Long term adaptation of lymphoma cells to hypoxia |
Description | This study explores the long-term adaptation mechanisms of lymphoma cells subjected to hypoxic conditions, with an emphasis on the HBL2 and Ramos cell lines under normoxia and 1% O2 environments. The research methodology encompassed lysing cells using a buffer containing sodium deoxycholate and TEAB, followed by protein quantification via a BCA assay. Subsequent steps involved protein digestion with trypsin, labelling with TMTpro™ 16plex Label Reagent Set for quantitative analysis, peptide fractionation, and LC-MS/MS analysis. The analytical process was supported by Proteome Discoverer 2.4 for protein identification and quantification. Experimental groups were categorized into "HBL2 normoxia," "HBL2 1% O2 adaptation," "Ramos normoxia," and "Ramos 1% O2 adaptation," conducted in triplicates to ensure reliability. This meticulous approach facilitated the delineation of unique proteomic landscapes indicative of hypoxia adaptation, unveiling cellular strategies employed by lymphoma cells to navigate low oxygen conditions. The findings advance our comprehension of how hypoxia influences cancer progression and potentially opens new avenues for targeting hypoxic niches within tumors. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-27 |
AnnouncementXML | Submission_2025-02-27_02:01:00.489.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matěj Běhounek |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-28 15:05:42 | ID requested | |
⏵ 1 | 2025-02-27 02:01:01 | announced | |
Publication List
Daumova L, Manakov D, Petrak J, Sovilj D, Behounek M, Andera L, Vit O, Souckova O, Havranek O, Dolnikova A, Renesova N, Tuskova L, Winkowska L, Bettazova N, Kupcova K, Kalbacova MH, Sikorova M, Trneny M, Klener P, Long-term adaptation of lymphoma cell lines to hypoxia is mediated by diverse molecular mechanisms that are targetable with specific inhibitors. Cell Death Discov, 11(1):65(2025) [pubmed] |
10.1038/s41420-025-02341-y; |
Keyword List
submitter keyword: Lymphoma cells (HBL2, TMT Proteomic analysis,Hypoxia adaptation, Ramos), Cellular mechanisms |
Contact List
prof. MUDr. Pavel Klener, Ph.D. |
contact affiliation | Institute of Pathological Physiology First Faculty of Medicine, Charles University |
contact email | pavel.klener@gmail.com |
lab head | |
Matěj Běhounek |
contact affiliation | BIOCEV, First Faculty of Medicine, Charles University, Prague, Czech Republic |
contact email | matej.behounek@lf1.cuni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050227
- Label: PRIDE project
- Name: Long term adaptation of lymphoma cells to hypoxia