PXD050201

PXD050201 is an original dataset announced via ProteomeXchange.

Title	GSNOR enhances salt tolerance of Ulva prolifera by regulating endogenous S -nitrosylation
Description	High salinity is considered to be one of the major abiotic stress factors that are detrimental to algal growth and development. In order to protect itself from high salinity, U.prolifera can sense salinity stress and trigger signaling pathways to activate relevant physiological mechanisms, and can also release germ cells to form new individuals. However, the molecular mechanisms involved in the level of protein s -nitrosylation in response to salt stress in macroalgae remain largely unknown.In this study, we performed quantitative proteomics analysis of protein modifications and identified a total of 1,620 proteins modified by S-nitrosylation in U.prolifera, in which a variety of antioxidant enzymes, including APX, CAT, and GR were up-regulated at the level of S-nitrosylation in response to salt stress. Further in vivo and in vitro validation results indicated that protein s-nitrosylation plays a positive role in enhancing antioxidant enzyme activities under short-term salt stress in U.prolifera. In addition, we explored the level of s-nitrosylation of key proteins associated with spore formation in U.prolifera, which will further improve our understanding of the molecular mechanisms of NO signaling and provide preliminary insights into the regulatory role of NO in U.prolifera spore formation.
HostingRepository	iProX
AnnounceDate	2024-02-27
AnnouncementXML	Submission_2024-02-27_18:04:02.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongren Zhang
SpeciesList	scientific name: Ulva prolifera; NCBI TaxID: 3117;
ModificationList	(13)C labeled residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-27 18:03:42	ID requested
⏵ 1	2024-02-27 18:04:02	announced

Dataset with its publication pending

submitter keyword: Ulva prolifera, GSNOR, S-nitrosylation, Salt stress

Songdong Shen
contact affiliation	Soochow University
contact email	shensongdong@suda.edu.cn
lab head
Dongren Zhang
contact affiliation	Soochow University
contact email	20214221086@stu.suda.edu.cn
dataset submitter

iProX dataset URI