PXD050193

PXD050193 is an original dataset announced via ProteomeXchange.

Title	MS-analysis of pull downs to elucidate putative transcriptional regulators from phasin and depolymerase genes
Description	LC-MS/MS analysis of promoter pull-down assays with crude protein extracts from R. eutropha Re2058/pCB113, to identify putative transcriptional regulators involved in the expression control of PHA metabolism, specifically targeting phasin phaP1 and depolymerase phaZ3 and phaZ5 genes.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:39:44.520.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050193
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Forbrig
SpeciesList	scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: 381666;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-27 10:22:17	ID requested
⏵ 1	2024-10-17 02:39:45	announced

10.1016/j.jbc.2024.107523;

10.6019/PXD050193;

Santolin L, Eichenroth RSJ, Cornehl P, Wortmann H, Forbrig C, Schulze A, Haq IU, Brantl S, Rappsilber J, Riedel SL, Neubauer P, Gimpel M, Elucidating regulation of polyhydroxyalkanoate metabolism in Ralstonia eutropha: Identification of transcriptional regulators from phasin and depolymerase genes. J Biol Chem, 300(8):107523(2024) [pubmed]

submitter keyword: LC-MSMS, Ralstonia eutropha

Juri Rappsilber
contact affiliation	Chair of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany
contact email	juri.rappsilber@tu-berlin.de
lab head
Christian Forbrig
contact affiliation	Technische Universität Berlin
contact email	c.forbrig@tu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050193

PRIDE project URI

• PRIDE
  1. PXD050193
     1. Label: PRIDE project
     2. Name: MS-analysis of pull downs to elucidate putative transcriptional regulators from phasin and depolymerase genes