PXD050178

PXD050178 is an original dataset announced via ProteomeXchange.

Title	A prokaryotic E2 enzyme imitates the ubiquitin pathway to regulate the antiviral cGAS
Description	The cyclic oligonucleotide-based anti-phage signalling system (CBASS) is a typeof innate prokaryotic immune system. Composed of a cyclic GMP-AMP synthase (cGAS) and CBASS-associated proteins, CBASS utilizes cyclic oligonucleotides to activate antiviral immunity. One major class of CBASS contains a homolog of eukaryotic ubiquitin-conjugating enzymes, which is either an E1-E2 fusion or a single E2. However, the functions of single E2s in CBASS remain elusive. Here, we report that a bacterial E2 enzyme regulates cGAS by imitating the ubiquitination cascade. This includes the processing of the cGAS C-terminus, conjugation of cGAS to a cysteine residue, ligation of cGAS to a lysine residue, cleavage of the isopeptide bond, and poly-cGASylation. The poly-cGASylation activates cGAS to produce cGAMP, which acts as an antiviral signal and leads to cell death. Thus, our findings reveal a unique regulatory role of E2 in CBASS.
HostingRepository	iProX
AnnounceDate	2024-02-27
AnnouncementXML	Submission_2024-05-09_20:55:04.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Xian
SpeciesList	scientific name: Serratia marcescens LCT-SM213; NCBI TaxID: 1162294;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 23:27:23	ID requested
1	2024-02-26 23:27:42	announced
⏵ 2	2024-05-09 20:55:05	announced	2024-05-10: Update information.

Yan Y, Xiao J, Huang F, Xian W, Yu B, Cheng R, Wu H, Lu X, Wang X, Huang W, Li J, Oyejobi GK, Robinson CV, Wu H, Wu D, Liu X, Wang L, Zhu B, Phage defence system CBASS is regulated by a prokaryotic E2 enzyme that imitates the ubiquitin pathway. Nat Microbiol, 9(6):1566-1578(2024) [pubmed]

submitter keyword: cGAS, cGASylation, CABSS

Xiaoyun Liu
contact affiliation	Peking University Health Science Center
contact email	xiaoyun.liu@bjmu.edu.cn
lab head
Wei Xian
contact affiliation	Peking University Health Science Center
contact email	xianwei@pku.edu.cn
dataset submitter

iProX dataset URI