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PXD050178

PXD050178 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA prokaryotic E2 enzyme imitates the ubiquitin pathway to regulate the antiviral cGAS
DescriptionThe cyclic oligonucleotide-based anti-phage signalling system (CBASS) is a typeof innate prokaryotic immune system. Composed of a cyclic GMP-AMP synthase (cGAS) and CBASS-associated proteins, CBASS utilizes cyclic oligonucleotides to activate antiviral immunity. One major class of CBASS contains a homolog of eukaryotic ubiquitin-conjugating enzymes, which is either an E1-E2 fusion or a single E2. However, the functions of single E2s in CBASS remain elusive. Here, we report that a bacterial E2 enzyme regulates cGAS by imitating the ubiquitination cascade. This includes the processing of the cGAS C-terminus, conjugation of cGAS to a cysteine residue, ligation of cGAS to a lysine residue, cleavage of the isopeptide bond, and poly-cGASylation. The poly-cGASylation activates cGAS to produce cGAMP, which acts as an antiviral signal and leads to cell death. Thus, our findings reveal a unique regulatory role of E2 in CBASS.
HostingRepositoryiProX
AnnounceDate2024-02-27
AnnouncementXMLSubmission_2024-05-09_20:55:04.335.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWei Xian
SpeciesList scientific name: Serratia marcescens LCT-SM213; NCBI TaxID: 1162294;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-26 23:27:23ID requested
12024-02-26 23:27:42announced
22024-05-09 20:55:05announced2024-05-10: Update information.
Publication List
Yan Y, Xiao J, Huang F, Xian W, Yu B, Cheng R, Wu H, Lu X, Wang X, Huang W, Li J, Oyejobi GK, Robinson CV, Wu H, Wu D, Liu X, Wang L, Zhu B, Phage defence system CBASS is regulated by a prokaryotic E2 enzyme that imitates the ubiquitin pathway. Nat Microbiol, 9(6):1566-1578(2024) [pubmed]
Keyword List
submitter keyword: cGAS, cGASylation, CABSS
Contact List
Xiaoyun Liu
contact affiliationPeking University Health Science Center
contact emailxiaoyun.liu@bjmu.edu.cn
lab head
Wei Xian
contact affiliationPeking University Health Science Center
contact emailxianwei@pku.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI