PXD050178
PXD050178 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A prokaryotic E2 enzyme imitates the ubiquitin pathway to regulate the antiviral cGAS |
Description | The cyclic oligonucleotide-based anti-phage signalling system (CBASS) is a typeof innate prokaryotic immune system. Composed of a cyclic GMP-AMP synthase (cGAS) and CBASS-associated proteins, CBASS utilizes cyclic oligonucleotides to activate antiviral immunity. One major class of CBASS contains a homolog of eukaryotic ubiquitin-conjugating enzymes, which is either an E1-E2 fusion or a single E2. However, the functions of single E2s in CBASS remain elusive. Here, we report that a bacterial E2 enzyme regulates cGAS by imitating the ubiquitination cascade. This includes the processing of the cGAS C-terminus, conjugation of cGAS to a cysteine residue, ligation of cGAS to a lysine residue, cleavage of the isopeptide bond, and poly-cGASylation. The poly-cGASylation activates cGAS to produce cGAMP, which acts as an antiviral signal and leads to cell death. Thus, our findings reveal a unique regulatory role of E2 in CBASS. |
HostingRepository | iProX |
AnnounceDate | 2024-02-27 |
AnnouncementXML | Submission_2024-05-09_20:55:04.335.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Xian |
SpeciesList | scientific name: Serratia marcescens LCT-SM213; NCBI TaxID: 1162294; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-26 23:27:23 | ID requested | |
1 | 2024-02-26 23:27:42 | announced | |
⏵ 2 | 2024-05-09 20:55:05 | announced | 2024-05-10: Update information. |
Publication List
Yan Y, Xiao J, Huang F, Xian W, Yu B, Cheng R, Wu H, Lu X, Wang X, Huang W, Li J, Oyejobi GK, Robinson CV, Wu H, Wu D, Liu X, Wang L, Zhu B, Phage defence system CBASS is regulated by a prokaryotic E2 enzyme that imitates the ubiquitin pathway. Nat Microbiol, 9(6):1566-1578(2024) [pubmed] |
Keyword List
submitter keyword: cGAS, cGASylation, CABSS |
Contact List
Xiaoyun Liu | |
---|---|
contact affiliation | Peking University Health Science Center |
contact email | xiaoyun.liu@bjmu.edu.cn |
lab head | |
Wei Xian | |
contact affiliation | Peking University Health Science Center |
contact email | xianwei@pku.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |