PXD050176

PXD050176 is an original dataset announced via ProteomeXchange.

Title	Outer membrane vesicles and associated proteins from the methanotrophic bacterium, Methylomicrobium album BG8
Description	Outer Membrane Vesicles (OMVs) are spherical bodies derived from the outer membrane of Gram-negative bacteria. Fully encapsulated by membrane, these extracellular structures transport cargo, often proteins, from within the bacterium into the external environment. Although there are multiple studies of OMVs released by pathogenic bacteria, the biogenesis and physiological role of OMVs released by environmental microorganisms is less understood. From a TEM screening of 8 methanotrophs affiliated with both Alpha- and Gammaproteobacteria, OMVs were only detected around cells of Methylomicrobium album BG8. OMVs were formed regardless of carbon or nitrogen source, amount of trace metal availability, or growth phase. The size and morphology of M. album BG8 OMVs were similar well-characterized OMVs from other Gram-negative bacteria. The OMVs were isolated from M. album BG8 cultures and their associated proteome revealed an abundance of membrane-associated proteins, transporters related to calcium uptake, and proteins related to Type I secretion systems (T1SS), such as TolC and RTX, suggesting mediation of cargo selection by the T1SS. This information provides clues to the biogenesis pathway and physiological function of OMVs in M. album BG8.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-25_15:26:31.501.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Methylomicrobium album BG8; NCBI TaxID: NEWT:686340;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 21:42:50	ID requested
⏵ 1	2026-05-25 15:26:32	announced

Dataset with its publication pending

submitter keyword: Methanotrophs, LC-MS

Richard Glen Uhrig
contact affiliation	Department oF Biological Sciences, University of Alberta
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050176
     1. Label: PRIDE project
     2. Name: Outer membrane vesicles and associated proteins from the methanotrophic bacterium, Methylomicrobium album BG8