PXD050173

PXD050173 is an original dataset announced via ProteomeXchange.

Title	Electron-Activated Dissociation and Collision-Induced Dissociation Glycopeptide Fragmentation for Improved Glycoproteomics
Description	Protein glycosylation is a critical PTM for the stability and biological function of many proteins, but full characterisation of site-specific glycosylation of proteins remains analytically challenging. Collision induced dissociation (CID) is the most common fragmentation method used in LC-MS/MS workflows, but loss of labile modifications render CID inappropriate for detailed characterisation of site-specific glycosylation. Electron-based dissociation (ExD) methods provide alternatives that retain intact glycopeptide fragments for unambiguous site localisation, but these methods often underperform CID due to increased reaction times and reduced efficiency. Electron activated dissociation (EAD) is another strategy for glycopeptide fragmentation. Here, we use a ZenoTOF 7600 SCIEX instrument to compare the performance of various fragmentation techniques for the analysis of a complex mixture of mammalian O- and N-glycopeptides.
HostingRepository	PRIDE
AnnounceDate	2024-07-04
AnnouncementXML	Submission_2024-07-03_19:56:16.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kyle Macauslane
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 19:41:44	ID requested
⏵ 1	2024-07-03 19:56:16	announced

10.1021/ACS.ANALCHEM.4C01450;

submitter keyword: CID,Glycopeptide, EAD, ZenoTOF 7600

Benjamin L Schulz
contact affiliation	School of Chemistry and Molecular Biosciences, University of Queensland, Australia
contact email	b.schulz@uq.edu.au
lab head
Kyle Macauslane
contact affiliation	School of Chemistry and Molecular Biosciences, University of Queensland
contact email	k.macauslane@uq.net.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050173

PRIDE project URI

• PRIDE
  1. PXD050173
     1. Label: PRIDE project
     2. Name: Electron-Activated Dissociation and Collision-Induced Dissociation Glycopeptide Fragmentation for Improved Glycoproteomics