PXD050160

PXD050160 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic characterization of astrocyte-derived extracellular vesicles under hypoxia
Description	This study investigated the role of astrocyte-derived EVs in neuroprotection and tissue recovery post-ischemia. Cortical astrocytes were cultured under normoxic and hypoxic conditions to harvest extracellular vesicles (EVs). The EVs were then administered to rats that had undergone experimental stroke. We assessed infarct volume, neuronal survival, and neurological function, and utilized label-free mass spectrometry-based proteomics to evaluate the hypoxia-induced changes in the EV proteome. Administration of astrocyte-derived EVs significantly reduced infarct volume, improved neurological function, and mitigated blood-brain barrier (BBB) permeability. Proteomic analysis revealed a differential expression of 67 upregulated proteins under hypoxic conditions and 37 downregulated proteins under normoxic conditions, highlighting the protective protein signatures elicited by astrocytes. Our findings underscore the role of astrocyte-derived EVs in fostering a conducive environment for neuronal survival under hypoxic stress. (Supported by DGAPA-PAPIIT IN207020 and IN214723 and CONACYT A1-S-13219).
HostingRepository	PRIDE
AnnounceDate	2025-06-15
AnnouncementXML	Submission_2025-06-14_16:09:02.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuel Rios-Castro
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	amidated residue; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	SYNAPT G2-Si

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 14:41:01	ID requested
⏵ 1	2025-06-14 16:09:02	announced

Publication List

10.2174/011570159X359837250611052037;

10.2174/011570159X359837250611052037;

Keyword List

submitter keyword: exosomes, proteomics, stroke, mass spectrometry,Extracellular vesicles, label-free MS, neuroprotection, LC-MS/MS

submitter keyword: exosomes, proteomics, stroke, mass spectrometry,Extracellular vesicles, label-free MS, neuroprotection, LC-MS/MS

Contact List

Luis B. Tovar-y-Romo
contact affiliation	Departamento de Neuropatología Molecular. Instituto de Fisiología Celular. Universidad Nacional Autónoma de México (UNAM)
contact email	ltovar@ifc.unam.mx
lab head
Emmanuel Rios-Castro
contact affiliation	Cinvestav-IPN
contact email	eriosc@cinvestav.mx
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD050160
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD050160

PRIDE project URI

Repository Record List

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