PXD050148

PXD050148 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Modulation of multistep cytokine induction in the activated microglia cell line MG5
Description	Ocular cytokine network plays pivotal role for initiation and progression of retinal degeneration, and microglia participate ocular inflammation. LPS activates MG5, mouse microglial cell line, and protein synthesis inhibitor CHX modulates cytokine expression pattern. To reveal comprehensive transition of molecular events, we performed proteome analysis using MG5 cells, and found that various mRNA modifying molecules are induced after LPS stimulation in addition to the inflammatory related molecules. The expression of mRNA modifying molecules are suppressed significantly by CHX, suggesting that transient induction of the molecules plays roles for determine the phenotype of inflammation in the microglia.
HostingRepository	jPOST
AnnounceDate	2025-02-26
AnnouncementXML	Submission_2025-02-25_07:00:04.592.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takeshi Kawamura
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 05:52:40	ID requested
⏵ 1	2025-02-25 07:00:04	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: LPS, CSF2, cycloheximide, microglia, retina

submitter keyword: LPS, CSF2, cycloheximide, microglia, retina

Contact List

Sumiko Watanabe
lab head
Takeshi Kawamura
contact affiliation	Tokyo University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002962/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002962/