PXD050142 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mapping the IMiD-dependent cereblon interactome using BioID-proximity labelling |
Description | This project employs the bacterial biotin-ligase, BioID2, to biotinylate and purify proteins that interact with cereblon (CRBN), including those that interact in a transient and/or weak fashion. In this project, cells expressing the fusion protein BioID2-CRBN are exposed to IMiDs (immunomodulatory imide drugs) and/or a proteasome inhibitor (bortezomib), and proteins that are biotinylated are subsequently purified and processed for mass spectrometry. Proteomic analysis identifies proteins that are purified in the different conditions, allowing to define how the CRBN interactome changes in the presence of these commonly used anti-myeloma drugs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:49:14.207.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Dagley |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-26 03:00:06 | ID requested | |
1 | 2024-07-08 19:09:58 | announced | |
⏵ 2 | 2024-10-22 06:49:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: IMiDs,multiple myeloma, cereblon, proximity labelling, BioID |
Contact List
ProfessorJake Shortt |
contact affiliation | Head of Haematology Research, School of Clinical Sciences, Monash University, Australia |
contact email | jake.shortt@monash.edu |
lab head | |
Laura Dagley |
contact affiliation | WEHI |
contact email | dagley.l@wehi.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050142 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050142
- Label: PRIDE project
- Name: Mapping the IMiD-dependent cereblon interactome using BioID-proximity labelling