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PXD050142

PXD050142 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMapping the IMiD-dependent cereblon interactome using BioID-proximity labelling
DescriptionThis project employs the bacterial biotin-ligase, BioID2, to biotinylate and purify proteins that interact with cereblon (CRBN), including those that interact in a transient and/or weak fashion. In this project, cells expressing the fusion protein BioID2-CRBN are exposed to IMiDs (immunomodulatory imide drugs) and/or a proteasome inhibitor (bortezomib), and proteins that are biotinylated are subsequently purified and processed for mass spectrometry. Proteomic analysis identifies proteins that are purified in the different conditions, allowing to define how the CRBN interactome changes in the presence of these commonly used anti-myeloma drugs.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:49:14.207.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Dagley
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-26 03:00:06ID requested
12024-07-08 19:09:58announced
22024-10-22 06:49:14announced2024-10-22: Updated project metadata.
Publication List
10.1111/FEBS.17196;
Keyword List
submitter keyword: IMiDs,multiple myeloma, cereblon, proximity labelling, BioID
Contact List
ProfessorJake Shortt
contact affiliationHead of Haematology Research, School of Clinical Sciences, Monash University, Australia
contact emailjake.shortt@monash.edu
lab head
Laura Dagley
contact affiliationWEHI
contact emaildagley.l@wehi.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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