PXD050142

PXD050142 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mapping the IMiD-dependent cereblon interactome using BioID-proximity labelling
Description	This project employs the bacterial biotin-ligase, BioID2, to biotinylate and purify proteins that interact with cereblon (CRBN), including those that interact in a transient and/or weak fashion. In this project, cells expressing the fusion protein BioID2-CRBN are exposed to IMiDs (immunomodulatory imide drugs) and/or a proteasome inhibitor (bortezomib), and proteins that are biotinylated are subsequently purified and processed for mass spectrometry. Proteomic analysis identifies proteins that are purified in the different conditions, allowing to define how the CRBN interactome changes in the presence of these commonly used anti-myeloma drugs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:49:14.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 03:00:06	ID requested
1	2024-07-08 19:09:58	announced
⏵ 2	2024-10-22 06:49:14	announced	2024-10-22: Updated project metadata.

Publication List

10.1111/FEBS.17196;

10.1111/FEBS.17196;

Keyword List

submitter keyword: IMiDs,multiple myeloma, cereblon, proximity labelling, BioID

submitter keyword: IMiDs,multiple myeloma, cereblon, proximity labelling, BioID

Contact List

ProfessorJake Shortt
contact affiliation	Head of Haematology Research, School of Clinical Sciences, Monash University, Australia
contact email	jake.shortt@monash.edu
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050142
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050142

PRIDE project URI

Repository Record List

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