PXD050132

PXD050132 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Data-Independent Acquisition Represents a Promising Alternative for Fast Photochemical Oxidation of Proteins (FPOP) Samples Analysis
Description	We propose a novel approach for FPOP data analysis, utilizing DIA data. The HbHp protein complex was analyzed by FPOP and measured on timsToF SCP in DIA, DDA and MS modes. The IDs of modified peptides were quantified for each acquisition mode and the extent of modification was calculated on the level of peptides. The reproducibility was evaluated by coefficients of variation. This work was mainly financially supported by the Czech Science Foundation (22-27695S), the Technology Agency of the Czech Republic (ODEEP-EU TH86010001), the Ministry of Education, Youth and Sports of the Czech Republic grant PHOTOMACHINES - Photosynthetic cell redesign for high yields of therapeutic peptides (CZ.02.01.01/00/22_008/0004624) and the Academy of Sciences of the Czech Republic (RVO: 61388971).
HostingRepository	PRIDE
AnnounceDate	2024-07-08
AnnouncementXML	Submission_2024-07-08_00:18:24.732.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050132
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marek Zakopcanik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF SCP

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 00:07:05	ID requested
⏵ 1	2024-07-08 00:18:25	announced

Publication List

10.1021/ACS.ANALCHEM.4C01084;
10.6019/PXD050132;

10.1021/ACS.ANALCHEM.4C01084;

10.6019/PXD050132;

Keyword List

submitter keyword: Fast Photochemical Oxidation of Proteins (FPOP), protein structure,protein footprinting, spectral library, hemoglobin-haptoglobin, data-independent acquisition (DIA)

submitter keyword: Fast Photochemical Oxidation of Proteins (FPOP), protein structure,protein footprinting, spectral library, hemoglobin-haptoglobin, data-independent acquisition (DIA)

Contact List

Petr Novak
contact affiliation	Institute of Microbiology, Czech Academy of Science, v.v.i.
contact email	pnovak@biomed.cas.cz
lab head
Marek Zakopcanik
contact affiliation	Institute of Microbiology of the CAS, v. v. i.
contact email	marek.zakopcanik@biomed.cas.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050132
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050132

PRIDE project URI

Repository Record List

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