PXD050118

PXD050118 is an original dataset announced via ProteomeXchange.

Title	Systematic Proteome Profiling of Maternal Plasma for Development of Preeclampsia Biomarkers
Description	Background: Preeclampsia (PE) is a hypertensive disorder of pregnancy with various clinical symptoms. However, traditional markers for the disease including high blood pressure and proteinuria are poor indicators of the related adverse outcomes. Here, we performed systematic proteome profiling of plasma samples obtained from pregnant women with PE to identify clinically effective diagnostic biomarkers. Methods: Proteome profiling was performed using TMT-based liquid chromatography-mass spectrometry (LC-MS/MS) followed by subsequent verification by multiple reaction monitoring (MRM) analysis on normal and PE maternal plasma samples. Functional annotations of differentially expressed proteins (DEPs) in PE were predicted using bioinformatic tools. The diagnostic accuracies of the biomarkers for PE were estimated according to the area under the receiver operating characteristics curve (AUC). Results: A total of 1,307 proteins were identified, and 870 proteins of them were quantified from plasma samples. Significant differences were evident in 138 DEPs, including 71 upregulated DEPs and 67 downregulated DEPs in the PE group, compared with those in the control group. Up-regulated proteins were significantly associated with biological processes including platelet degranulation, proteolysis, lipoprotein metabolism, and cholesterol efflux. Biological processes including blood coagulation and acute-phase response were enriched for down-regulated proteins. Of these, 40 proteins were subsequently validated in an independent cohort of 26 PE patients and 29 healthy controls. APOM, LCN2, and QSOX1 showed high diagnostic accuracies for PE detection (AUC > 0.9 and P < 0.001, for all) as validated by MRM and ELISA. Conclusions: Our data demonstrate that three plasma biomarkers, identified by systematic proteomic profiling, present a possibility for the assessment of PE, independent of the clinical characteristics of pregnant women.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:26:56.907.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050118
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jaemin Lim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-25 09:06:33	ID requested
⏵ 1	2025-05-06 13:26:57	announced

Lim JH, Lim JM, Lee HM, Lee HJ, Kwak DW, Han YJ, Kim MY, Jung SH, Kim YR, Ryu HM, Kim KP, Systematic Proteome Profiling of Maternal Plasma for Development of Preeclampsia Biomarkers. Mol Cell Proteomics, 23(9):100826(2024) [pubmed]

10.1016/j.mcpro.2024.100826;

10.6019/PXD050118;

submitter keyword: proteomics, plasma, preeclampsia,human

Kwang Pyo Kim
contact affiliation	Department of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin, Republic of KOREA
contact email	kimkp@khu.ac.kr
lab head
Jaemin Lim
contact affiliation	Kyung Hee Univiersity
contact email	jami1031@khu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050118
     1. Label: PRIDE project
     2. Name: Systematic Proteome Profiling of Maternal Plasma for Development of Preeclampsia Biomarkers