PXD050108

PXD050108 is an original dataset announced via ProteomeXchange.

Title	ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair
Description	Mono-ubiquitinated PCNA (mono-Ub-PCNA) is generated when replication forks stall and facilitates the DNA lesion bypass process. After resolving a replication stall, Ub-PCNA needs to be de-ubiquitinated to resume high-fidelity DNA synthesis. ATAD5 cooperates with UAF1-USP1 to de-ubiquitinate mono-Ub-PCNA. However, it remains unclear how Ub-PCNA de-ubiquitination is regulated in a timely manner. We found that BAZ1B, a regulatory subunit of the chromatin-remodeling complex, fine-tunes de-ubiquitination of Ub-PCNA. The BAZ1B binding region of ATAD5 surrounds the ATAD5 UAF1-binding domain. Abrogation of the ATAD5-BAZ1B interaction leads to premature de-ubiquitination of Ub-PCNA after hydrogen peroxide treatment. BAZ1B-binding defective ATAD5 cells are more sensitive to oxidative stress compared to wild-type cells. These results suggest that BAZ1B inhibits premature Ub-PCNA de-ubiquitination to maintain genome integrity.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:11:08.422.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050108
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Byung-gyu kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-24 16:49:01	ID requested
⏵ 1	2025-05-06 13:11:09	announced

10.6019/PXD050108;

10.1038/s41467-024-55005-3;

submitter keyword: BAZ1B, de-ubiquitination, DNA repair, USP1, SMARCA5, ATAD5,BAZ1B, UAF1, PCNA

Sukhyun Kang
contact affiliation	Center for Genomic Integrity, Institute for Basic Science, Ulsan 44919, Republic of Korea
contact email	kangsh@ibs.re.kr
lab head
Byung-gyu kim
contact affiliation	Center for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact email	goldenlion@ibs.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050108

PRIDE project URI

• PRIDE
  1. PXD050108
     1. Label: PRIDE project
     2. Name: ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair