PXD050097

PXD050097 is an original dataset announced via ProteomeXchange.

Title	BS3 crosslinking of the Anaphase-Promoting Complex/Cyclosome (APC/C)
Description	In order to investigate the dynamic regulation of the APC/C by intrinsically disordered regions (IDRs) upon phosphorylation, we employed cross-linking mass spectrometry (CLMS). Given that the phosphorylation of Apc1-300L (IDR in Apc1) is a key determinant in its release from the co-activator binding site, we compared the interaction profiles of unphosphorylated and hyper-phosphorylated rAPC/C_WT complexes. The CLMS analysis highlights not only known interactions consistently identified within APC/C complexes, regardless of phosphorylation status, but also dynamic IDR-mediated interactions that are lost upon phosphorylation or are phosphorylation-dependent. Additionally, the data indicate that phosphorylation significantly reduces Apc1-300L's interaction with Apc8-L (IDR in Apc8) and Apc6, suggesting Apc1-300L's dislocation from the co-activator Cdc20 binding site, thereby facilitating Cdc20 loading onto the APC/C.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:12.226.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 19:10:45	ID requested
1	2024-04-23 15:55:10	announced
⏵ 2	2024-10-22 06:37:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Crosslinking mass spectrometry, the Anaphase-Promoting Complex/Cyclosome (APC/C)

Juri Rappsilber
contact affiliation	Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050097
     1. Label: PRIDE project
     2. Name: BS3 crosslinking of the Anaphase-Promoting Complex/Cyclosome (APC/C)