PXD050086

PXD050086 is an original dataset announced via ProteomeXchange.

Title	Identification of potential interaction partners of the CoxG homologs Saci_1119 and Saci_2031 in Sulfolobus acidocaldarius MW001
Description	This project aims on the study of glycerol metabolism in the thermoacidophilic crenarchaeal model organism Sulfolobus acidocaldarius, previously regarded as a non-glycerol utilizer. On the one hand, changes in the proteome of the glycerol-adapted strain Sulfolobus acidocaldarius MW00G upon growth with different concentrations of glycerol (10 mM, 20 mM, and 40 mM) as sole carbon and energy source was investigated. On the other hand, potential interaction partners of the two carbon monoxide dehydrogenase subunit G (CoxG) homologs, Saci_1119 and Saci_2031, were identified by overexpression of C-terminally HA-tagged versions of the two CoxG proteins and subsequent co-immunoprecipitation, thereby aiming to elucidate their potential role as membrane anchors for the glycerol-3-phosphate dehydrogenase (G3PDH) from the respective gene cluster.
HostingRepository	PRIDE
AnnounceDate	2025-03-18
AnnouncementXML	Submission_2025-03-18_02:15:14.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Sulfolobus acidocaldarius; NCBI TaxID: 2285;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 07:35:46	ID requested
⏵ 1	2025-03-18 02:15:14	announced

Dataset with its publication pending

submitter keyword: CoxG,S. acidocaldarius

glycerole degradation

Farnusch Kaschani
contact affiliation	Farnusch Kaschani, Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD050086

PRIDE project URI

• PRIDE
  1. PXD050086
     1. Label: PRIDE project
     2. Name: Identification of potential interaction partners of the CoxG homologs Saci_1119 and Saci_2031 in Sulfolobus acidocaldarius MW001