PXD050073

PXD050073 is an original dataset announced via ProteomeXchange.

Title	Pre-existing cell subpopulations in primary prostate cancer tumors display surface fingerprints of docetaxel-resistant cells
Description	Docetaxel resistance presents a significant obstacle in the treatment of prostate cancer (PCa), resulting in unfavorable patient prognoses. Intratumoral heterogeneity, often associated with epithelial-to-mesenchymal transition (EMT), has previously emerged as a phenomenon facilitating adaptations to various stimuli, thus promoting cancer cell diversity and eventually resistance to chemotherapy, including docetaxel. Hence, comprehending intratumoral heterogeneity is essential for better patient prognosis and the development of personalized treatment strategies. To address this, we employed a high-throughput single-cell flow cytometry approach to identify a specific surface fingerprint associated with docetaxel-resistance in PCa cells and complement it with protein composition analysis of extracellular vesicles We further performed validation of selected antigens using docetaxel-resistant patient-derived xenografts in vivo and determine a 6-molecule surface fingerprint associated with docetaxel resistance in primary PCa specimens. Remarkably, we observed consistent overexpression of CD95 and SSEA-4 surface antigens in both in vitro and in vivo docetaxel-resistant models and in a cell subpopulation of primary PCa tumors exhibiting EMT features. Furthermore, CD95, along with the essential enzymes involved in SSEA-4 synthesis, ST3GAL1, and ST3GAL2, displayed a significant increase in PCa patients undergoing docetaxel-based therapy, correlating with poor survival outcomes. In conclusion, we demonstrate that the identified 6-molecule surface fingerprint associated with docetaxel resistance pre-exists in a subpopulation of primary PCa tumors before docetaxel treatment. This fingerprint, therefore, deserves further validation as a promising tool for predicting docetaxel response in PCa patients before therapy initiation.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:21:14.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katerina Hanakova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 03:08:01	ID requested
⏵ 1	2025-05-06 13:21:15	announced

10.1007/s13402-024-00982-2;

Dr, á, pela S, Kvoka, č, kov, á B, Slab, á, kov, á E, Kotrbov, á A, G, ö, m, ö, ryov, á K, Fedr R, Kurf, ü, rstov, á D, Eli, á, š M, Š, tudent V, Len, č, é, š, ov, á F, Ranjani GS, Posp, í, chalov, á V, Bryja V, van Weerden WM, Puhr M, Culig Z, Bouchal J, Sou, č, ek K, Pre-existing cell subpopulations in primary prostate cancer tumors display surface fingerprints of docetaxel-resistant cells. Cell Oncol (Dordr), 48(1):205-218(2025) [pubmed]

submitter keyword: prostate cancer, plasticity, docetaxel-resistance, SSEA-4, intratumoral heterogeneity, CD95/Fas

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Katerina Hanakova
contact affiliation	CEITEC MU
contact email	katerina.hanak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050073

PRIDE project URI

• PRIDE
  1. PXD050073
     1. Label: PRIDE project
     2. Name: Pre-existing cell subpopulations in primary prostate cancer tumors display surface fingerprints of docetaxel-resistant cells