PXD050049 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic studies of human placentas reveal partnerships associated with preeclampsia, diabetes, gravidity, and labor |
Description | VEGFR2 (Vascular endothelial growth factor receptor 2) is a central regulator of placental angiogenesis. The study of the VEGFR2 proteome of chorionic villi at term revealed its partners MDMX (Double minute 4 protein) and PICALM (Phosphatidylinositol-binding clathrin assembly protein). Subsequently, the oxytocin receptor (OT-R) and vasopressin V1aR receptor were detected in MDMX and PICALM immunoprecipitations. Immunogold electron microscopy showed VEGFR2 on endothelial cell (EC) nuclei, mitochondria, and Hofbauer cells (HC), tissue-resident macrophages of the placenta. MDMX, PICALM, and V1aR were located on EC plasma membranes, nuclei, and HC nuclei. Unexpectedly, PICALM and OT-R were detected on EC projections into the fetal lumen and OT-R on 20-150 nm clusters therein, prompting the hypothesis that placental exosomes transport OT-R to the fetus and across the blood-brain barrier. Insights on gestational complications were gained by univariable and multivariable regression analyses associating preeclampsia with lower MDMX protein levels in membrane extracts of chorionic villi, and lower MDMX, PICALM, OT-R, and V1aR with spontaneous vaginal deliveries compared to cesarean deliveries before the onset of labor. We found select associations between higher MDMX, PICALM, OT-R protein levels and either gravidity, diabetes, BMI, maternal age, or neonatal weight, and correlations only between PICALM-OT-R (p<2.7x10-8), PICALM-V1aR (p<0.006), and OT-R-V1aR (p<0.001). These results offer for exploration new partnerships in metabolic networks, tissue-resident immunity, and labor, notably for HC that predominantly express MDMX. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:41:07.883.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dale Chaput |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-22 16:45:45 | ID requested | |
1 | 2024-05-22 02:50:59 | announced | |
⏵ 2 | 2024-10-22 06:41:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
Ho SJ, Chaput D, Sinkey RG, Garces AH, New EP, Okuka M, Sang P, Arlier S, Semerci N, Steffensen TS, Rutherford TJ, Alsina AE, Cai J, Anderson ML, Magness RR, Uversky VN, Cummings DAT, Tsibris JCM, with preeclampsia, diabetes, gravidity, and labor. Cell Commun Signal, 22(1):221(2024) [pubmed] |
10.1186/s12964-024-01567-0; |
Keyword List
submitter keyword: VEGFR2, Placenta, Hofbauer cells, MDMX, TRIM21, PICALM, Oxytocin receptor, Proteomics |
Contact List
John Tsibris |
contact affiliation | Professor, Department of Obstetrics and Gynecology, University of South Florida, Tampa, Florida, USA |
contact email | tsibris@usf.edu |
lab head | |
Dale Chaput |
contact affiliation | University of South Florida |
contact email | chaput@usf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD050049 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050049
- Label: PRIDE project
- Name: Proteomic studies of human placentas reveal partnerships associated with preeclampsia, diabetes, gravidity, and labor