PXD050042

PXD050042 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MEF2A interactome in striated muscle by an affinity-purification followed by LC-MS-MS
Description	Myocyte Enhancer Factor 2 (MEF2) proteins are involved in multiple developmental, physiological, and pathological processes in vertebrates. Protein:protein interactions underlie the plethora of biological processes impacted by MEF2A, necessitating a detailed characterization of the MEF2A interactome. A nanobody based affinity-purification/mass spectrometry strategy was employed to achieve this goal. Specifically, the MEF2A protein complexes were captured from myogenic lysates using a GFP-tagged MEF2A protein immobilized with a GBP-nanobody followed by LC-MS/MS proteomic analysis to identify MEF2A interactors.
HostingRepository	PRIDE
AnnounceDate	2024-02-27
AnnouncementXML	Submission_2024-02-27_07:58:44.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Declan Williams
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-22 09:30:18	ID requested
⏵ 1	2024-02-27 07:58:44	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: MEF2A, C2C12, affinity-purification

submitter keyword: MEF2A, C2C12, affinity-purification

Contact List

John C McDermott
contact affiliation	Department of Biology, York University, Toronto, ON M3J 1P3, Canada
contact email	jmcderm@yorku.ca
lab head
Declan Williams
contact affiliation	Dalriada Drug Discovery
contact email	gwillym.williams@dalriadatx.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD050042
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD050042

PRIDE project URI

Repository Record List

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