PXD050034

PXD050034 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	High-throughput nano-flow proteomics using a dual-column electrospray source
Description	With current trends in proteomics, especially regarding clinical and low input (to single cell) samples, it is increasingly important to both maximize the throughput of the analysis and maintain as much sensitivity as possible. The new generation of mass spectrometers (MS) are taking a huge leap in sensitivity, allowing to analyze samples with shorter liquid chromatography (LC) methods while digging as deep in the proteome. However, the throughput can be doubled by implementing a dual column nano-LC-MS configuration. For this purpose, we used a dual-column setup with a two-outlet electrospray source, and compared it to a classic dual-column setup with a single-outlet source.
HostingRepository	PRIDE
AnnounceDate	2024-04-22
AnnouncementXML	Submission_2024-04-22_07:14:16.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	An Staes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-22 05:36:10	ID requested
⏵ 1	2024-04-22 07:14:17	announced

Publication List

10.1021/ACS.ANALCHEM.4C00845;

10.1021/ACS.ANALCHEM.4C00845;

Keyword List

submitter keyword: DIA,nanoLC, tandem setup, high-throughput

submitter keyword: DIA,nanoLC, tandem setup, high-throughput

Contact List

Simon Devos
contact affiliation	VIB Proteomics Core, Technologiepark-Zwijnaarde 75,B905Ghent, Belgium VIB Center for Medical Biotechnology, Technologiepark-Zwijnaarde 75, B9052 Ghent, Belgium Department of Biomolecular Medicine, Ghent University, Technologiepark-Zwijnaarde 75, B9052 Ghent, Belgium
contact email	simon.devos@vib-ugent.be
lab head
An Staes
contact affiliation	Medical Protein Chemistry
contact email	an.staes@vib-ugent.be
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050034
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050034

PRIDE project URI

Repository Record List

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